Ordering Recommendation

Useful for general testing in contexts of compliance and/or abuse. Preferred test to follow-up presumptive results.




Quantitative Liquid Chromatography-Tandem Mass Spectrometry


Mon, Thu, Sat


1-7 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Random urine.

Specimen Preparation

Transfer 2 mL urine with no additives or preservatives to an ARUP Standard Transport Tube. (Min: 1 mL)

Storage/Transport Temperature


Unacceptable Conditions

Room temperature specimens.


Ambient: Unacceptable; Refrigerated: 3 weeks; Frozen: 3 months

Reference Interval

Drugs Covered Methylphenidate
Methylphenidate 10 ng/mL
Ritalinic acid 50 ng/mL

Interpretive Data

Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Positive Cutoff:   Methylphenidate: 10 ng/mL
                    Ritalinic acid: 50 ng/mL   
The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, diluted/adulterated urine, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


Hotline History


CPT Codes

80360 (Alt code: G0480)


Component Test Code* Component Chart Name LOINC
2003116 Methylphenidate, Urn, Quant 3809-1
2003117 Ritalinic acid, Urn, Quant 33507-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Attenade
  • Concerta
  • Daytrana
  • Dexmethylphenidate
  • Focalin (Methylphenidate and Metabolite - Confirmation/Quantitation - Urine)
  • Metadate
  • Methylin
  • Methylphenidate
  • Ritalin
  • Ritalinic Acid
  • Urine ritalin concentration
Methylphenidate and Metabolite, Urine, Quantitative