Ordering Recommendation

Quantify kappa and lambda light chains in paraffin. Includes interpretation.

Mnemonic
K/L ISH
Methodology

In situ hybridization (ISH)

Performed

Mon-Fri

Reported

2-5 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Tissue.

Specimen Preparation

Formalin fix (10% neutral buffered formalin) and paraffin-embed tissue. Transport tissue block or 10 unstained 5 micron slides in a tissue transport kit (ARUP supply #47808). Available online through eSupply using ARUP ConnectTM or contact ARUP Client Services at (800) 522-2787. (Min: 5 slides) Protect paraffin block and/or slides from excessive heat.

Storage/Transport Temperature

Room temperature or refrigerated. Ship in cooled container during summer months.

Unacceptable Conditions

Specimens fixed or processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives (B-4 or B-5). Frozen specimens.

Remarks

Include surgical pathology report.

Stability

Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable

Reference Interval
Available Separately Test Name Reference Interval
No Kappa by in situ Hybridization, Paraffin By report
No Lambda by in situ Hybridization, Paraffin By report
No Kappa/Lambda Ratio 0.5-5.0

Interpretive Data

Refer to report.

Compliance Category

Analyte Specific Reagent (ASR)

Note
Hotline History
N/A
CPT Codes

88368; 88369

Components
Component Test Code* Component Chart Name LOINC
2002887 Kappa/Lambda ISH Comment
2002999 ISH Tissue Source 31208-2
2003000 ISH Reference Number 30896-5
2003001 H and E Slide Description 19772-3
2003218 Kappa/Lambda ISH Clonality
2003219 Kappa/Lambda ISH Ratio
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • K/L ISH
  • Kappa and Lambda ISH
  • Kappa and Lambda mRNA Probes
Kappa/Lambda Light Chain Panel by in situ Hybridization, Paraffin