Ordering Recommendation

Quantify kappa and lambda light chains in paraffin. Includes interpretation.


In situ hybridization (ISH)




2-5 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation


Specimen Preparation

Formalin fix (10% neutral buffered formalin) and paraffin-embed tissue. Transport tissue block or 10 unstained 5 micron slides in a tissue transport kit (ARUP supply #47808). Available online through eSupply using ARUP ConnectTM or contact ARUP Client Services at (800) 522-2787. (Min: 5 slides) Protect paraffin block and/or slides from excessive heat.

Storage/Transport Temperature

Room temperature or refrigerated. Ship in cooled container during summer months.

Unacceptable Conditions

Specimens fixed or processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives (B-4 or B-5). Frozen specimens.


Include surgical pathology report.


Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable

Reference Interval
Available Separately Test Name Reference Interval
No Kappa by in situ Hybridization, Paraffin By report
No Lambda by in situ Hybridization, Paraffin By report
No Kappa/Lambda Ratio 0.5-5.0

Interpretive Data

Refer to report.

Compliance Statement A: For laboratory developed tests using a manufacturer labeled ASR as the reagent providing the specificity of the assay. Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

Hotline History
CPT Codes

88368; 88369

Component Test Code* Component Chart Name LOINC
2002887 Kappa/Lambda ISH Comment
2002999 ISH Tissue Source 31208-2
2003000 ISH Reference Number 30896-5
2003001 H and E Slide Description 19772-3
2003218 Kappa/Lambda ISH Clonality
2003219 Kappa/Lambda ISH Ratio
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • K/L ISH
  • Kappa and Lambda ISH
  • Kappa and Lambda mRNA Probes
Kappa/Lambda Light Chain Panel by in situ Hybridization, Paraffin