Ordering Recommendation

Most comprehensive test to aid in the diagnosis and management of monoclonal gammopathies, including multiple myeloma. Use to detect, quantify, and characterize serum monoclonal protein. 

Mnemonic
IFE FLC
Methodology

Qualitative Immunofixation Electrophoresis/Quantitative Capillary Electrophoresis/Quantitative Immunoturbidimetry/Quantitative Spectrophotometry

Performed

Sun-Sat

Reported

1-5 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 1.5 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma. Room temperature specimens.

Remarks
Stability

After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 1 month

Reference Interval
Test Number
Components
Reference Interval
0050640 Protein Electrophoresis, Serum Effective August 19, 2019
Test Number
Components
Reference Interval
  Total Protein, Serum Refer to report
  Albumin Refer to report
  Alpha 1 Globulin Refer to report
  Alpha 2 Globulin Refer to report
  Beta Globulin Refer to report
  Gamma Refer to report

0050340 Immunoglobulin A Effective February 16, 2016
0-30 days: 1-7 mg/dL
1 month: 1-53 mg/dL
2 months: 3-47 mg/dL
3 months: 5-46 mg/dL
4 months: 4-72 mg/dL
5 months: 8-83 mg/dL
6 months: 8-67 mg/dL
7-8 months: 11-89 mg/dL
9-11 months: 16-83 mg/dL
1 year: 14-105 mg/dL
2 years: 14-122 mg/dL
3 years: 22-157 mg/dL
4 years: 25-152 mg/dL
5-7 years: 33-200 mg/dL
8-9 years: 45-234 mg/dL
10 years and older: 68-408 mg/dL

0050350 Immunoglobulin G
0- 30 days: 611-1542 mg/dL              
1 month: 241-870 mg/dL                          
2 months: 198-577 mg/dL            
3 months: 169-558 mg/dL                    
4 months: 188-536 mg/dL                          
5 months: 165-781 mg/dL                          
6 months: 206-676 mg/dL                           
7-8 months: 208-868 mg/dL                        
9-11 months: 282-1026 mg/dL
1 year: 331-1164 mg/dL
2 years: 407-1009 mg/dL
3 years: 423-1090 mg/dL
4 years: 444-1187 mg/dL
5-7 years: 608-1229 mg/dL
8-9 years: 584-1509 mg/dL
10 years and older: 768-1632 mg/dL

0050355 Immunoglobulin M Effective February 16, 2016
0-30 days: 0-24 mg/dL                     
1 month: 19-83 mg/dL                                
2 months: 16-100 mg/dL                          
3 months: 23-85 mg/dL                               
4 months: 26-96 mg/dL                             
5 months: 31-103 mg/dL                            
6 months: 33-97 mg/dL                               
7-8 months: 32-120 mg/dL                         
9-11 months: 39-142 mg/dL
1 year: 41-164 mg/dL
2 years: 46-160 mg/dL
3 years: 45-190 mg/dL
4 years: 41-186 mg/dL
5-7 years: 46-197 mg/dL
8-9 years: 49-230 mg/dL
10 years and older: 35-263 mg/dL

  Kappa Qnt Free Light Chains Effective February 18, 2020
3.30 - 19.40 mg/L
  Lambda Qnt Free Light Chains Effective February 18, 2020
5.71-26.30 mg/L
  Kappa/Lambda Free Light Chain Ratio 0.26-1.65

Interpretive Data

Undetected antigen excess is a rare event but cannot be excluded. Free light chain results should always be interpreted in conjunction with other clinical and laboratory findings.

Compliance Category

FDA

Note

A copy of the graph will follow the final report.  This assay is highly sensitive to increasing concentrations of monoclonal free kappa or free lambda light chains in the serum of patients with evolving or relapsing myelomas.

Hotline History
N/A
CPT Codes

82784 x3; 84155; 84165; 86334; 83520 x2

Components
Component Test Code* Component Chart Name LOINC
0050020 Albumin 2862-1
0050035 Alpha 1 Globulin 2865-4
0050060 Alpha 2 Globulin 2868-8
0050110 Beta Globulin 2871-2
0050270 Gamma 2874-6
0050271 Immunofixation 25700-6
0050340 Immunoglobulin A 2458-8
0050350 Immunoglobulin G 2465-3
0050355 Immunoglobulin M 2472-9
0050545 Total Protein, Serum 2885-2
0055168 Kappa Qnt Free Light Chains 36916-5
0055169 Lambda Qnt Free Light Chains 33944-0
0055179 Kappa/Lambda Free Light Chain Ratio 80517-6
2005168 SPEP/IFE Interpretation 49275-1
2012457 EER Monoclonal Protein and FLC, Serum
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • IFE
  • IgA, IgG, and IgM
  • Immunofixation, serum
  • Kappa Qnt Free Light Chains
  • Kappa/Lambda Free Light Chain Ratio
  • Kappa/Lambda Free Light Chains
  • Lambda Qnt Free Light Chains
  • MGUS study
  • Monoclonal Protein Study
  • Mutiple Myeloma Study
  • SPEP
Monoclonal Protein Study, Expanded Panel, Serum