KIT Mutations, Melanoma
2002695
Ordering Recommendation
Detect activating mutations in KIT and PDGFRA. Predict response to tyrosine kinase inhibitor (TKI) therapy.
Mnemonic
KIT MELAN
Methodology
Massively Parallel Sequencing
Performed
Varies
Reported
10-12 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
- Patient Preparation
- Collect
- Tumor tissue.
- Specimen Preparation
- Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Diff-Quik and Papanicolaou stained cytology smears are also acceptable. Number of slides needed is dependent on the tumor cellularity of the smear. Slide(s) will be destroyed during testing process and will not be returned to client. Protect from excessive heat. Transport block and/or slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Resections: Transport 8 unstained 5-micron slides. (Min: 5 slides)
Small Biopsies: Transport 15 unstained 5-micron slides. (Min: 10 slides) - Storage/Transport Temperature
- Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.
- Unacceptable Conditions
- Less than 10 percent tumor. Specimens fixed/processed in heavy metal fixatives. Decalcified specimens. FNA smears with less than 50 tumor cells.
- Remarks
- Include surgical pathology report.
- Stability
- Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Reference Interval
By report
Interpretive Data
Refer to report.
Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
Note
A full list of the targeted genes and regions is listed in the Additional Technical Information.
Hotline History
View Hotline History
CPT Code(s)
81272; 88381; 81314
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 2002148 | Block ID | 57723-9 |
| 2002694 | KIT Mutation, Melanoma Interpretation | 55201-8 |
Aliases
- c-KIT
- CD117 KIT
- cKIT
- KIT exons 9, 11,13, 14, 17, and 18
- TKI resistance
- TKI sensitivity