JAK2 Exon 12 Mutation Analysis by PCR

2002357
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Example Reports
Not Detected
Positive

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Additional Technical Information

Ordering Recommendation

Most appropriate in cases of high suspicion of polycythemia vera with negative JAK2 V617F mutation status.

Mnemonic

JAK2 EX12

Methodology

Polymerase Chain Reaction

Performed

Varies

Reported

3-9 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Whole blood or bone marrow (EDTA). Also acceptable: DNA extracted by CLIA-certified lab.

Specimen Preparation

Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow: Do not freeze. Transport 3 mL bone marrow. (Min: 1 mL)
Extracted DNA: Transport 40 uL DNA with at least 50 ng/uL concentration. (Min: 40 uL) Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma, serum, FFPE tissue blocks/slides, or frozen tissue. Specimens collected in anticoagulants other than EDTA. Clotted or grossly hemolyzed specimens.

Remarks
Stability

Ambient: 24 hours; Refrigerated: 4 days; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerate: Indefinitely; Frozen: Indefinitely

Reference Interval

Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note

Hotline History

View Hotline History

 

Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
08/15/2022
X
05/16/2022
X
02/23/2022
X
N/A

CPT Codes

81279

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Components

Component Test Code* Component Chart Name LOINC
2002358 JAK2 Exon 12 Mutation Analysis by PCR 63421-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • JAK 2 exon 12
  • Janus Kinase 2 Gene
  • Janus Kinase 2 Gene Sequencing
  • MPN JAK2 testing
  • Tyrosine Kinase Gene Sequencing
JAK2 Exon 12 Mutation Analysis by PCR