Most appropriate in cases of high suspicion of polycythemia vera with negative JAK2 V617F mutation status.
Polymerase Chain Reaction
DNA isolation: Sun-Sat
Assay: Mon, Wed, Fri
Lavender (EDTA) or bone marrow (EDTA). Also acceptable: DNA extracted by CLIA certified lab.
Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow: Do not freeze. Transport 3 mL bone marrow. (Min: 1 mL)
Extracted DNA: Transport 40 uL DNA with at least 50 ng/uL concentration. (Min: 40 uL) Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Plasma, serum, FFPE tissue blocks/slides, or frozen tissue, DNA extracted by a non-CLIA lab. Specimens collected in anticoagulants other than EDTA or sodium heparin. Clotted or grossly hemolyzed specimens.
Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerate: Indefinitely; Frozen: Indefinitely
Refer to report.
Laboratory Developed Test (LDT)
|Component Test Code*||Component Chart Name||LOINC|
|2002358||JAK2 Exon 12 Mutation Analysis by PCR||63421-2|
- JAK 2 exon 12
- Janus Kinase 2 Gene
- Janus Kinase 2 Gene Sequencing
- MPN JAK2 testing
- Tyrosine Kinase Gene Sequencing