Most appropriate in cases of high suspicion of polycythemia vera with negative JAK2 V617F mutation status.
Polymerase Chain Reaction
DNA isolation: Sun-Sat
Assay: Mon, Wed, Fri
New York DOH Approval Status
Whole blood or bone marrow (EDTA). Also acceptable: DNA extracted by CLIA-certified lab.
Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow: Do not freeze. Transport 3 mL bone marrow. (Min: 1 mL)
Extracted DNA: Transport 40 uL DNA with at least 50 ng/uL concentration. (Min: 40 uL) Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Plasma, serum, FFPE tissue blocks/slides, or frozen tissue. Specimens collected in anticoagulants other than EDTA. Clotted or grossly hemolyzed specimens.
Ambient: 24 hours; Refrigerated: 4 days; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerate: Indefinitely; Frozen: Indefinitely
Refer to report.
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Laboratory Developed Test (LDT)
|Component Test Code*||Component Chart Name||LOINC|
|2002358||JAK2 Exon 12 Mutation Analysis by PCR||63421-2|
- JAK 2 exon 12
- Janus Kinase 2 Gene
- Janus Kinase 2 Gene Sequencing
- MPN JAK2 testing
- Tyrosine Kinase Gene Sequencing