Ordering Recommendation

Screen for 21-hydroxylase deficiency, the most common congenital adrenal insufficiency.

Mnemonic
CAH 21 HYDROX
Methodology

Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry

Performed

Sun-Sat

Reported

1-4 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Collect between 6-10 a.m.

Collect

Serum separator tube or green (sodium or lithium heparin). Also acceptable: Pink (K2EDTA).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1.8 mL serum or plasma to an ARUP Standard Transport Tube and freeze immediately. (Min: 0.9 mL)

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions

Refrigerated or room temperature specimens.

Remarks
Stability

After separation from cells: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 6 months

Reference Interval
Available Separately
Components
Reference Interval
2001638 Androstenedione Effective August 19, 2013
Age Female Male
Premature Infants, 26-28 weeks-Day 4 0.92-2.82 ng/mL 0.92-2.82 ng/mL
Premature Infants, 31-35 weeks-Day 4 0.80-4.46 ng/mL 0.80-4.46 ng/mL
Full-term Infants, 1-7 days 0.20-2.90 ng/mL 0.20-2.90 ng/mL
8-30 days 0.18-0.80 ng/mL 0.18-0.80 ng/mL
1-5 months 0.06-0.68 ng/mL 0.06-0.68 ng/mL
6-24 months Less than 0.15 ng/mL 0.03-0.15 ng/mL
2-3 years Less than 0.16 ng/mL Less than 0.11 ng/mL
4-5 years 0.02-0.21 ng/mL 0.02-0.17 ng/mL
6-7 years 0.02-0.28 ng/mL 0.01-0.29 ng/mL
8-9 years 0.04-0.42 ng/mL 0.03-0.30 ng/mL
10-11 years 0.09-1.23 ng/mL 0.07-0.39 ng/mL
12-13 years 0.24-1.73 ng/mL 0.10-0.64 ng/mL
14-15 years 0.39-2.00 ng/mL 0.18-0.94 ng/mL
16-17 years 0.35-2.12 ng/mL 0.30-1.13 ng/mL
18-39 years 0.26-2.14 ng/mL 0.33-1.34 ng/mL
40 years and older 0.13-0.82 ng/mL 0.23-0.89 ng/mL
Pre-menopausal 0.26-2.14 ng/mL Does Not Apply
Postmenopausal 0.13-0.82 ng/mL Does Not Apply
Tanner Stage I 0.05-0.51 ng/mL 0.04-0.32 ng/mL
Tanner Stage II 0.15-1.37 ng/mL 0.08-0.48 ng/mL
Tanner Stage III 0.37-2.24 ng/mL 0.14-0.87 ng/mL
Tanner Stage IV-V 0.35-2.05 ng/mL 0.27-1.07 ng/mL

0092332 17-Hydroxyprogesterone Quantitative by HPLC-MS/MS, Serum or Plasma Effective August 19, 2013
Age
Female
Male
Premature (26-28 weeks) 124-841 ng/dL 124-841 ng/dL
Premature (29-35 weeks) 26-568 ng/dL 26-568 ng/dL
Full term Day 3 7-77 ng/dL 7-77 ng/dL
4 days-30 days 7-106 ng/dL Less than 200 ng/dL
1 month-2 months 13-106 ng/dL Less than 200 ng/dL
3 months-5 months 13-106 ng/dL 3-90 ng/dL
6  months-1 year Less than or equal to 148 ng/dL Less than or equal to 148 ng/dL
2-3 years Less than or equal to 256 ng/dL Less than or equal to 228 ng/dL
4-6 years Less than or equal to 299 ng/dL Less than or equal to 208 ng/dL
7-9 years Less than or equal to 71 ng/dL Less than or equal to 63 ng/dL
10-12 years Less than or equal to 129 ng/dL Less than or equal to 79 ng/dL
13-15 years 9-208 ng/dL 9-140 ng/dL
16-17 years Less than or equal to 178 ng/dL 24-192 ng/dL
18 years and older Less than 207 ng/dL Less than 139 ng/dL
Follicular 15-70 ng/dL Does Not Apply
Luteal 35-290 ng/dL Does Not Apply
Tanner Stage I Less than or equal to 74 ng/dL Less than or equal to 62 ng/dL
Tanner Stage II Less than or equal to 164 ng/dL Less than or equal to 104 ng/dL
Tanner Stage III 13-209 ng/dL Less than or equal to 151 ng/dL
Tanner Stage IV-V 7-170 ng/dL 20-173 ng/dL

0092333 17-Hydroxypregnenolone Quantitative by LC-MS/MS, Serum or Plasma
Age
Female
Male
Premature (26-28 weeks) 1219-9799 ng/dL 1219-9799 ng/dL
Premature (29-36 weeks) 346-8911 ng/dL 346-8911 ng/dL
Full Term (1-5 months) 229-3104 ng/dL 229-3104 ng/dL
6-12 months less than or equal to 917ng/dL less than or equal to 917ng/dL
13-23 months less than or equal to 592 ng/dL less than or equal to 592 ng/dL
2-4 years less than or equal to 280 ng/dL less than or equal to 249 ng/dL
5-6 years less than or equal to 350 ng/dL less than or equal to 319 ng/dL
7-9 years less than or equal to 212 ng/dL less than or equal to 187 ng/dL
10-12 years less than or equal to 398 ng/dL less than or equal to 392 ng/dL
13-15 years less than or equal to 407 ng/dL 35-465 ng/dL
16-17 years less than or equal to 423 ng/dL 32-478 ng/dL
18 years and older Less than 226 ng/dL Less than 442 ng/dL
Tanner Stage I less than or equal to 235 ng/dL less than or equal to 208 ng/dL
Tanner Stage II less than or equal to 367 ng/dL less than or equal to 355 ng/dL
Tanner Stage III less than or equal to 430 ng/dL less than or equal to 450 ng/dL
Tanner Stage IV-V less than or equal to 412 ng/dL 35-478 ng/dL

2001640 Dehydroepiandrosterone, Serum or Plasma Effective August 19, 2013
Age Female Male
Premature Less than 40 ng/mL Less than 40 ng/mL
0-1 day Less than 11 ng/mL Less than 11 ng/mL
2-6 days Less than 8.7 ng/mL Less than 8.7 ng/mL
7 days-1 month Less than 5.8 ng/mL Less than 5.8 ng/mL
1-5 months Less than 2.9 ng/mL Less than 2.9 ng/mL
6-24 months Less than 1.9 9 ng/mL Less than 2.5 ng/mL
2-3 years Less than 0.85 ng/mL Less than 0.63 ng/mL
4-5 years Less than 1.03 ng/mL Less than 0.95 ng/mL
6-7 years Less than 1.79 ng/mL 0.06-1.93 ng/mL
8-9 years 0.14-2.35 ng/mL 0.10-2.08 ng/mL
10-11 years 0.43-3.78 ng/mL 0.32-3.08 ng/mL
12-13 years 0.89-6.21 ng/mL 0.57-4.10 ng/mL
14-15 years 1.22-7.01 ng/mL 0.93-6.04 ng/mL
16-17 years 1.42-9.00 ng/mL 1.17-6.52 ng/mL
18-39 years 1.33-7.78 ng/mL 1.33-7.78 ng/mL
40 years and older 0.63-4.70 ng/mL 0.63-4.70 ng/mL
Postmenopausal 0.60-5.73 ng/mL Does Not Apply
Tanner Stage I 0.14-2.76 ng/mL 0.11-2.37 ng/mL
Tanner Stage II 0.83-4.87 ng/mL 0.37-3.66 ng/mL
Tanner Stage III 1.08-7.56 ng/mL 0.75-5.24 ng/mL
Tanner Stage IV-V 1.24-7.88 ng/mL 1.22-6.73 ng/mL

Interpretive Data



Compliance Category

Laboratory Developed Test (LDT)

Note
Hotline History
N/A
CPT Codes

82157; 83498; 84143; 82626

Components
Component Test Code* Component Chart Name LOINC
0092332 17-Hydroxyprogesterone, HPLC-MS/MS 1668-3
0092333 17-Hydroxypregnenolone Quant, MS/MS, Ser 6765-2
2001639 Androstenedione by TMS 1854-9
2001641 Dehydroepiandrosterone by TMS 2193-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • 17-Hydroxypregnenolone (CAH 21 Hydroxylase Deficiency Panel)
  • 17-Hydroxyprogesterone (CAH 21 Hydroxylase Deficiency Panel)
  • Androstenedione (CAH 21 Hydroxylase Deficiency Panel)
  • Dehydroepiandrosterone (DHEA) (CAH 21 Hydroxylase Deficiency Panel)
Congenital Adrenal Hyperplasia Panel, 21-Hydroxylase Deficiency