Functional testing of red blood cell sensitivity to osmotic stress; do not use to distinguish between spherocytes in hereditary spherocytosis and acquired autoimmune hemolytic anemia.
Green (Sodium or Lithium Heparin) or Lavender (EDTA).
Transport 2 unfixed, air-dried, and unstained smears. (Min: 2 smears made from the blood submitted) AND 5 mL whole blood. (Min: 1 mL) Specimens should be refrigerated within 30 minutes after collection. Place both slides and whole blood specimens in an osmotic fragility transport kit (ARUP supply #53821) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Grossly hemolyzed specimens.
Ambient: Unacceptable; Refrigerated: 72 hours; Frozen: Unacceptable
Within normal curve limits.
For patients with acute hemolysis, a normal red cell osmotic fragility test result cannot exclude an osmotic fragility abnormality since the osmotically labile cells may be hemolyzed and not present. Recommend testing during a state of prolonged homeostasis with stable hematocrit.
|Component Test Code*||Component Chart Name||LOINC|
- FRAGILITY, OSMOTIC (RBC)
- Osmotic Fragility
- RBC Fragility, Erythrocytes
- Red Cell Fragility
- Spherocytic Hemolytic Disease