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Time Sensitive
Ordering Recommendation

Functional testing of red blood cell sensitivity to osmotic stress; do not use to distinguish between spherocytes in hereditary spherocytosis and acquired autoimmune hemolytic anemia.

Mnemonic
OSM FRG
Methodology

Spectrophotometry

Performed

Mon-Fri

Reported

1-5 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Green (Sodium or Lithium Heparin) or Lavender (EDTA).

Specimen Preparation

Transport 2 unfixed, air-dried, and unstained smears. (Min: 2 smears made from the blood submitted) AND 5 mL whole blood. (Min: 1 mL) Specimens should be refrigerated within 30 minutes after collection. Place both slides and whole blood specimens in an osmotic fragility transport kit (ARUP supply #53821) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Grossly hemolyzed specimens.

Remarks
Stability

Ambient: Unacceptable; Refrigerated: 72 hours; Frozen: Unacceptable

Reference Interval

Within normal curve limits.

Interpretive Data

For patients with acute hemolysis, a normal red cell osmotic fragility test result cannot exclude an osmotic fragility abnormality since the osmotically labile cells may be hemolyzed and not present. Recommend testing during a state of prolonged homeostasis with stable hematocrit.

No compliance statements are in use for this test.

Note
Hotline History
N/A
CPT Codes

85555

Components
Component Test Code* Component Chart Name LOINC
0049015 Osmotic Fragility 34964-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • FRAGILITY, OSMOTIC (RBC)
  • Osmotic Fragility
  • RBC Fragility, Erythrocytes
  • Red Cell Fragility
  • Spherocytic Hemolytic Disease
Osmotic Fragility, Erythrocyte