Ordering Recommendation

Acceptable reflexive screening test for celiac disease. Positive screen results reflex to IgA and IgG antibody testing for tissue transglutaminase antibody (tTG) and deaminated gliadin peptide antibody (DGP). Preferred screening test is Celiac Disease Reflexive Cascade (2008114) or Tissue Transglutaminase (tTG) Antibody, IgA (0097709) testing.


Semi-Quantitative Enzyme-Linked Immunosorbent Assay


Mon, Wed, Fri


1-6 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 1 mL)

Storage/Transport Temperature


Unacceptable Conditions

Plasma. Contaminated, hemolyzed, or grossly lipemic specimens.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year

Reference Interval

Test Number
Reference Interval
  Celiac Disease Dual Antigen Screen 0 to 19 Units

Interpretive Data

Refer to individual components.

Component Interpretation
Celiac Disease Dual Antigen Screen 19 Units or less

20 Units or greater
Negative - No significant level of detectable IgA or IgG antibodies
against human tissue transglutaminase or gliadin peptide.

Positive - Presence of IgA and/or IgG antibodies against human tissue
transglutaminase and/or gliadin peptide; suggests possibility of certain
gluten sensitive enteropathies such as celiac disease and dermatitis herpetiformis.

Compliance Category



The Celiac Disease Dual Antigen Screen is a combined antibody assay for tissue transglutaminase and deamidated gliadin-derived peptide antibodies, IgA and IgG.

If Celiac Disease Dual Antigen Screen is 20 Units or greater, then tTG IgA and Gliadin Peptide IgA will be added. If tTG IgA and Gliadin Peptide IgA are negative, tTG IgG and Gliadin Peptide IgG will be added. Additional charges apply.

Hotline History


CPT Codes

83516; if reflexed, add 86258 x2; 86364 x2


Component Test Code* Component Chart Name LOINC
0051691 Celiac Disease Dual Antigen Screen 63420-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Anti-tissue Transglutaminase Antibody
  • CD Dual Ag screen
  • Celiac Sprue
  • Deaminated Gliadin Peptide Antibodies
  • DGP
  • DGP IgA IgG
  • Dual Ag reflex screen
  • Dual antigen screen, Dual Ag screen
  • Gluten Sensitivity Screen
  • Gluten-Sensitive Enteropathy Tests
  • tTG IgG, IgA
Celiac Disease Dual Antigen Screen with Reflex