Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."
Contaminated, heat-inactivated, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
|Bordetella pertussis Antibody, IgM by ELISA||Effective February 20, 2018
0.9 IV or less: Negative - No significant level of detectable B. pertussis IgM antibody.
1.0-1.1 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.2 IV or greater: Positive - IgM antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.
|2004326||Bordetella pertussis Antibody, IgM by Immunoblot||
Recommend that treatment decisions be based on the result of the B. pertussis IgM immunoblot test instead of the ELISA test. B. pertussis IgM test by ELISA may produce false-positive results.
Laboratory Developed Test (LDT)
If Bordetella pertussis Antibody, IgM by ELISA is 1.2 IV or greater, then Bordetella pertussis IgM Immunoblot testing will be added. Additional charges apply.
86615; if reflexed, add 86615
|Component Test Code*||Component Chart Name||LOINC|
|2001783||B. pertussis Ab, IgM by ELISA||42329-3|
- B. pertussis IgM reflex
- Pertussis Antibody
- Pertussis IgM Ab ELISA reflex to immunoblot