Ordering Recommendation

Evaluation of IgM pertussis antibodies has little clinical utility. CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR (0065080) and/or Bordetella pertussis Culture (0060117).

New York DOH Approval Status

This test is not New York state approved. There are no New York state-approved laboratories available. Submit a Non-Permitted Laboratory Request Form (NPL) to the NYDOH prior to collection of specimen. If NPL is approved by NYDOH, and sample is received at ARUP, testing will be performed.

Specimen Required

Patient Preparation

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature


Unacceptable Conditions

Bacterially contaminated, heat-inactivated, hemolyzed, icteric, lipemic, or turbid specimens.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)


Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot




1-8 days

Reference Interval

Test Number
Reference Interval
  B. pertussis Ab, IgM by ELISA 1.1 IV or less

Interpretive Data

Recommend that treatment decisions be based on the result of the B. pertussis IgM immunoblot test instead of the ELISA test.  B. pertussis IgM test by ELISA may produce false-positive results.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Component Interpretaion
Bordetella pertussis Antibody, IgM by ELISA 0.9 IV or less: Negative - No significant level of detectable B. pertussis IgM antibody.
1.0-1.1 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.2 IV or greater: Positive - IgM antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.

Compliance Category

Laboratory Developed Test (LDT)


If Bordetella pertussis Antibody, IgM by ELISA is 1.2 IV or greater, then Bordetella pertussis IgM Immunoblot testing will be added. Additional charges apply.

Hotline History


CPT Codes

86615; if reflexed, add 86615


Component Test Code* Component Chart Name LOINC
2001783 B. pertussis Ab, IgM by ELISA 42329-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • B. pertussis IgM reflex
  • Pertussis Antibody
  • Pertussis IgM Ab ELISA reflex to immunoblot
Bordetella pertussis Antibody, IgM by ELISA with Reflex to Immunoblot