May be used as evidence of vaccination or past infection; test does not determine immunity to B. pertussis. CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR (0065080) and/or Bordetella pertussis Culture (0060117). In most cases, serology testing is not recommended for the diagnosis of active pertussis infection.
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."
Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
|Bordetella pertussis Antibody IgG by ELISA||Effective February 20, 2018
0.94 IV or less: Negative - No significant level of detectable B. pertussis IgG antibody.
0.95-1.04 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.05 IV or greater: Positive - IgG antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.
|2004327||Bordetella pertussis Antibody, IgG by Immunoblot||
Refer to report.
Laboratory Developed Test (LDT)
If Bordetella pertussis Antibody, IgG by ELISA is 1.05 IV or greater, then Bordetella pertussis IgG Immunoblot testing will be added. Additional charges apply.
86615; if reflexed, add 86615
|Component Test Code*||Component Chart Name||LOINC|
|2001782||B. pertussis Ab, IgG by ELISA||42330-1|
- B. pertussis IgG reflex
- Pertussis Antibody
- Pertussis IgG reflex to immunoblot