Ordering Recommendation

May be used to provide evidence of vaccination or past infection; test does not determine immunity to B. pertussis. CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR (0065080) and/or Bordetella pertussis Culture (0060117). In most cases, serology testing is not recommended for the diagnosis of active pertussis infection.

New York DOH Approval Status

Testing is not New York state approved. Specimens from New York clients will be sent out to a New York state-approved laboratory.

Specimen Required

Patient Preparation

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."
New York State Clients: 0.5 mL (Min: 0.25 mL)

Storage/Transport Temperature


Unacceptable Conditions

Bacterially contaminated, heat-inactivated, hemolyzed, icteric, lipemic, or turbid specimens.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
New York State Clients: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 1 month


Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot


Tue, Fri


1-5 days

Reference Interval

Test Number
Reference Interval
  B. pertussis Ab, IgG by ELISA 1.04 IV or less

Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Component Interpretation
Bordetella pertussis Antibody IgG by ELISA 0.94 IV or less: Negative - No significant level of detectable B. pertussis IgG antibody.
0.95-1.04 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.05 IV or greater: Positive - IgG antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.

Compliance Category

Laboratory Developed Test (LDT)


If Bordetella pertussis Antibody, IgG by ELISA is 1.05 IV or greater, then Bordetella pertussis IgG Immunoblot testing will be added. Additional charges apply.

Hotline History


CPT Codes

86615; if reflexed, add 86615


Component Test Code* Component Chart Name LOINC
2001782 B. pertussis Ab, IgG by ELISA 42330-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • B. pertussis IgG reflex
  • Pertussis Antibody
  • Pertussis IgG reflex to immunoblot
Bordetella pertussis Antibody, IgG by ELISA with Reflex to Immunoblot