Ordering Recommendation

Use to identify causal F8 gene intron 22A or intron 1 variant in individuals with established severe hemophilia A and determine carrier status of those with relatives with a known inversion of intron 1 or 22A. The most comprehensive test for individuals with severe hemophilia A is Hemophilia A (F8) 2 Inversions with Reflex to Sequencing and Reflex to Deletion/Duplication (3004232). For mild to moderate hemophilia A, Hemophilia A (F8) Sequencing (3004241) is recommended.




Inverse Polymerase Chain Reaction/Electrophoresis




7-14 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B).

Specimen Preparation

Transport 3 mL whole blood. (Min: 1 mL)

Storage/Transport Temperature


Unacceptable Conditions

Room temperature: 7 days; Refrigerated: 1 month; Frozen: Unacceptable

Reference Interval

Interpretive Data

Background Information for Hemophilia A (F8) 2 Inversions:
Severe deficiency of factor VIII clotting activity leading to spontaneous joint or deep muscle bleeding.  Moderate to mild deficiency is associated with prolonged bleeding after tooth extractions, surgery, or injuries and recurrent or delayed wound healing.
Incidence: 1 in 4,000-5,000 live male births worldwide, rare in females.
Inheritance: X-linked recessive. Of simplex cases, 85 percent of mothers are carriers and 10-15 percent of boys have a de novo mutation.
Penetrance: 100 percent in males and 10 percent in females.
Cause: Deleterious F8 gene mutations.
Clinical Sensitivity: 51 percent of mutations causing severe hemophilia A are detected by F8 inversion testing. This assay does not detect F8 mutations associated with mild or moderate hemophilia A in males.
Methodology: Intron 22-A and intron 1 inversions detected by inverse PCR and electrophoresis.
Analytical Sensitivity and Specificity: 99 percent.
Limitations: Diagnostic errors can occur due to rare sequence variations. F8 mutations, other than the F8 intron 22-A and intron 1 inversions, will not be detected.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Counseling and informed consent are recommended for genetic testing. Consent forms are available online.

Compliance Category

Laboratory Developed Test (LDT)


Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
2001761 Hemophilia A (F8) Inversions Interp 82343-5
2001762 F8 Inv Specimen 66746-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • DNA Analysis for Hemophilia A
  • F8
  • Carrier Detection and Prenatal Diagnosis
  • Carrier Detection of Hemophilia A
  • F8 Inversion
  • Factor VIII Deficiency
  • Factor VIII Inversion
  • Factor VIII Inversions
  • Hemophilia A
  • Hemophilia A (Factor VIII) Inversions
  • Severe Hemophilia A
Hemophilia A (F8) 2 Inversions