Ordering Recommendation

Determine antiviral drug resistance by DNA sequencing.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Lavender (EDTA), pink (K2EDTA), serum separator tube, or plasma preparation tube.

Specimen Preparation

Separate serum or plasma from cells within 24 hours. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature


Unacceptable Conditions

Heparinized specimens.


Ambient: 72 hours; Refrigerated: 1 week; Frozen: 6 weeks


Polymerase Chain Reaction/Sequencing


Tue, Fri


7-10 days

Reference Interval


Interpretive Data

Both the HBV RT polymerase and the HBsAg encoding regions are sequenced. Resistance and surface antigen mutations are reported. In addition, the major HBV genotypes are identified. Mutations in viral sub-populations below 20 percent of total may not be detected.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


This test may be unsuccessful if the HBV viral load is less than log 3.0 or 1,000 IU/mL of plasma.

Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
2001568 HBV Surface Antigen Mutations by Seq 32366-7
2001569 Hepatitis B Genotype by Seq 32366-7
2001570 HBV RT Polymerase Mutations by Seq 49883-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Adefovir
  • Entecavir
  • HBsAg mutations
  • HBV drug resistance testing
  • HBV Genotype
  • HBV Genotyping
  • HBV Resistance
  • HBV Resistance Sequencing
  • HBV Sequencing
  • Hepatitis B Drug Resistance Panel
  • Lamivudine
  • Telbivudine
  • Tenofovir
Hepatitis B Virus Genotype by Sequencing