0099592
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Jo-1 Antibody, IgG

ANTI-JO
Example Reports
Negative
Positive

Ordering Recommendation

Recommended first-line test for the evaluation of polymyositis or inflammatory myopathies.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube or red tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP standard transport tube. (Min: 0.2 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma or other body fluids.

Remarks
Stability

After separation from the cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Methodology

Semi-Quantitative Multiplex Bead Assay

Performed

Sun-Sat

Reported

1-3 days

Reference Interval

Test Number
 Components
 Reference Interval
  Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG 40 AU/mL or less

Interpretive Data

Presence of Jo-1 (antihistidyl transfer RNA [t-RNA] synthetase) antibody is associated with polymyositis and may also be seen in patients with dermatomyositis. Jo-1 antibody is associated with pulmonary involvement (interstitial lung disease), Raynaud phenomenon, arthritis, and mechanic's hands (implicated in antisynthetase syndrome).


Component
 Interpretation
Jo-1 Antibody, IgG 29 AU/mL or less            Negative
30-40 AU/mL                   Equivocal
41 AU/mL or greater      Positive

Compliance Category

FDA

Note

Presence of Jo-1 antibody is found in patients with pure polymyositis, pure dermatomyositis, or myositis associated with another rheumatic disease or with interstitial lung disease.

Hotline History

View Hotline History

 

Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
07/21/2025
X
N/A

CPT Codes

86235

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Components

Component Test Code* Component Chart Name LOINC
0099592 Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG 33571-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Autoantibodies to Jo 1 Antigen
  • Synthetase Antibody
  • Jo 1 Antibodies
  • Histidyl-tRNA Synthetase Antibodies
  • Anti-Jo-1 Antibody
  • Antibodies to Jo 1 Antigen
  • Polymyositis Antibodies
Jo-1 Antibody, IgG