Ordering Recommendation

Recommended first-line test for the evaluation of polymyositis or inflammatory myopathies.

Mnemonic
ANTI-JO
Methodology

Semi-Quantitative Multiplex Bead Assay

Performed

Sun-Sat

Reported

1-2 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tubes.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma or other body fluids.

Remarks
Stability

After separation from the cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval
29 AU/mL or less Negative
30-40 AU/mL Equivocal
41 AU/mL or greater Positive

Interpretive Data

Presence of Jo-1 (antihistidyl transfer RNA [t-RNA] synthetase) antibody is associated with polymyositis and may also be seen in patients with dermatomyositis. Jo-1 antibody is associated with pulmonary involvement (interstitial lung disease), Raynaud phenomenon, arthritis, and mechanic's hands (implicated in antisynthetase syndrome).

Compliance Category

FDA

Note

Presence of Jo-1 antibody is found in patients with pure polymyositis, pure dermatomyositis, or myositis associated with another rheumatic disease or with interstitial lung disease.

Hotline History
N/A
CPT Codes

86235

Components
Component Test Code* Component Chart Name LOINC
0099592 Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG 33571-1
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Aliases
  • Anti-Jo-1 Antibody
  • Antibodies to Jo 1 Antigen
  • Autoantibodies to Jo 1 Antigen
  • Histidyl-tRNA Synthetase Antibodies
  • Jo 1 Antibodies
  • Polymyositis Antibodies
  • Synthetase Antibody
Jo-1 Antibody, IgG