Ordering Recommendation

Recommended first-line test for the evaluation of polymyositis or inflammatory myopathies.

Mnemonic
ANTI-JO
Methodology

Semi-Quantitative Multiplex Bead Assay

Performed

Sun-Sat

Reported

1-2 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tubes.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma or other body fluids.

Remarks
Stability

After separation from the cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval
29 AU/mL or less Negative
30-40 AU/mL Equivocal
41 AU/mL or greater Positive

Interpretive Data

Presence of Jo-1 (antihistidyl transfer RNA [t-RNA] synthetase) antibody is associated with polymyositis and may also be seen in patients with dermatomyositis. Jo-1 antibody is associated with pulmonary involvement (interstitial lung disease), Raynaud phenomenon, arthritis, and mechanic's hands (implicated in antisynthetase syndrome).

No compliance statements are in use for this test.

Note

Presence of Jo-1 antibody is found in patients with pure polymyositis, pure dermatomyositis, or myositis associated with another rheumatic disease or with interstitial lung disease.

Hotline History
N/A
CPT Codes

86235

Components
Component Test Code* Component Chart Name LOINC
0099592 Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG 33571-1
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Aliases
  • Anti-Jo-1 Antibody
  • Antibodies to Jo 1 Antigen
  • Autoantibodies to Jo 1 Antigen
  • Histidyl-tRNA Synthetase Antibodies
  • Jo 1 Antibodies
  • Polymyositis Antibodies
  • Synthetase Antibody
Jo-1 Antibody, IgG