Use in conjunction with positive serologic testing for the workup of suspected acute Lyme neuroborreliosis. Do not order in the absence of clinical symptoms.
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Sun - Sat
Transfer 3 mL CSF to an ARUP Standard Transport Tube. (Min: 0.5 mL)
Contaminated, heat-inactivated, or hemolyzed specimens.
Ambient: 8 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
|0.99 LIV or less||Negative - Antibody to B. burgdorferi not detected.|
|1.00-1.20 LIV||Equivocal - Repeat testing in 10-14 days may be helpful.|
|1.21 LIV or greater||Positive - Probable presence of antibody to B. burgdorferi detected.|
The detection of antibodies to B. burgdorferi in cerebrospinal fluid may indicate central nervous system infection. However, consideration must be given to possible contamination by blood or transfer of serum antibodies across the blood-brain barrier.
Current CDC recommendations for the serologic diagnosis of Lyme disease are to screen with a polyvalent ELISA test and confirm equivocal and positive results with immunoblot. Both IgM and IgG immunoblots should be performed on samples less than 4 weeks after appearance of erythema migrans. Only IgG immunoblot should be performed on samples greater than 4 weeks after the disease onset. IgM immunoblot in the chronic stage is not recommended and does not aid in the diagnosis of neuroborreliosis or chronic Lyme disease. Please submit requests for appropriate immunoblot testing within 10 days.
Once this test is performed, if:
a) Negative - no further testing is done.
b) Positive or equivocal - Immunoblot testing will be performed on the original sample upon receiving a request. Sample will be held for 30 days only.
|Component Test Code*||Component Chart Name||LOINC|
|0099483||Borrelia burgdorferi Abs, ELISA, CSF||6318-0|
- Lyme Antibodies, Total
- Lyme Disease
- Lyme Disease Ab CSF, ELISA