Ordering Recommendation

Rule-out Cushing syndrome.

Mnemonic

CORT UF

Methodology

Quantitative Liquid Chromatography-Tandem Mass Spectrometry

Performed

Sun-Sat

Reported

1-4 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

24-hour or random urine. Refrigerate 24-hour specimen during collection.

Specimen Preparation

Transport one 4 mL aliquot of urine. (Min: 1 mL) Record total volume and collection time interval on transport tube and test request form.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Room temperature specimens. Acidified specimens or specimens with preservatives.

Remarks
Stability

Ambient: Unacceptable; Refrigerated: 2 weeks; Frozen: 6 months

Reference Interval

Effective April 7, 2014

Test Name
Female
Male
Cortisol, Urine Free - per 24h 3-8 years: Less than or equal to 18 µg/24h
9-12 years: Less than or equal to 37 µg/24h
13-17 years: Less than or equal to 56 µg/24h
18 years and older: Less than or equal to 45 µg/24h
3-8 years: Less than or equal to 18 µg/24h
9-12 years: Less than or equal to 37 µg/24h
13-17 years: Less than or equal to 56 µg/24h
18 years and older: Less than or equal to 60 µg/24h
Cortisol, Urine Free - ratio to CRT Prepubertal: Less than 25 µg/g CRT
18 years and older: Less than 24 µg/g CRT
Pregnancy: Less than 59 µg/g CRT
Prepubertal: Less than 25 µg/g CRT
18 years and older: Less than 32 µg/g CRT

Interpretive Data

Per 24h calculations are provided to aid interpretation for collections with a duration of 24 hours and an average daily urine volume. For specimens with notable deviations in collection time or volume, ratios of analytes to a corresponding urine creatinine concentration may assist in result interpretation.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note

Reference intervals based on literature from Taylor R.L. et al., Validation of a High-Throughput Liquid Chromatography-Tandem Mass Spectrometry Method for Urine Cortisol and Cortisone. Clinical Chemistry 2002; 48:1511-1519.

Hotline History

N/A

CPT Codes

82530

Components

Component Test Code* Component Chart Name LOINC
0020207 Creatinine, Urine - per volume 2161-8
0020208 Creatinine, Urine - per 24h 2162-6
0092087 Cortisol, Urine Free - per volume 34909-2
0093444 Cortisol, Urine Free - ratio to CRT 32009-3
0097110 Total Volume 19153-6
0097111 Hours Collected 30211-7
0097224 Cortisol, Urine Free - per 24h 2147-7
2006309 Cortisol, Urine Interpretation 48767-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Cortisol, Free, LC/MS/MS, Second Void Urine
  • Urinary Free Cortisol
Cortisol Urine Free by LC-MS/MS