Ordering Recommendation

Rule-out Cushing syndrome.

Mnemonic
CORT UF
Methodology

Quantitative Liquid Chromatography-Tandem Mass Spectrometry

Performed

Sun-Sat

Reported

1-4 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

24-hour or random urine. Refrigerate 24-hour specimen during collection.

Specimen Preparation

Transport one 4 mL aliquot of urine. (Min: 1 mL) Record total volume and collection time interval on transport tube and test request form.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Room temperature specimens. Acidified specimens or specimens with preservatives.

Remarks
Stability

Ambient: Unacceptable; Refrigerated: 2 weeks; Frozen: 6 months

Reference Interval

Effective April 7, 2014

Test Name
Female
Male
Cortisol, Urine Free - per 24h 3-8 years: Less than or equal to 18 µg/24h
9-12 years: Less than or equal to 37 µg/24h
13-17 years: Less than or equal to 56 µg/24h
18 years and older: Less than or equal to 45 µg/24h
3-8 years: Less than or equal to 18 µg/24h
9-12 years: Less than or equal to 37 µg/24h
13-17 years: Less than or equal to 56 µg/24h
18 years and older: Less than or equal to 60 µg/24h
Cortisol, Urine Free - ratio to CRT Prepubertal: Less than 25 µg/g CRT
18 years and older: Less than 24 µg/g CRT
Pregnancy: Less than 59 µg/g CRT
Prepubertal: Less than 25 µg/g CRT
18 years and older: Less than 32 µg/g CRT

Interpretive Data

Interpretive Data: The optimal specimen for this testing is a 24-hour urine collection. Mass per day calculations are not reported for the following specimen types: a random collection, a collection with duration of less than 20 hours, a collection with duration of greater than 28 hours, or a collection with total volume less than 400 mL or greater than 5000 mL. Ratios to creatinine may be useful for these evaluations.

Baseline urinary free cortisol excretion less than 5 ug/d may be consistent with adrenal insufficiency.

Compliance Category

Laboratory Developed Test (LDT)

Note

Reference intervals based on literature from Taylor R.L. et al., Validation of a High-Throughput Liquid Chromatography-Tandem Mass Spectrometry Method for Urine Cortisol and Cortisone. Clinical Chemistry 2002; 48:1511-1519.

Hotline History
N/A
CPT Codes

82530

Components
Component Test Code* Component Chart Name LOINC
0020207 Creatinine, Urine - per volume 2161-8
0020208 Creatinine, Urine - per 24h 2162-6
0092087 Cortisol, Urine Free - per volume 34909-2
0093444 Cortisol, Urine Free - ratio to CRT 32009-3
0097110 Total Volume 19153-6
0097111 Hours Collected 30211-7
0097224 Cortisol, Urine Free - per 24h 2147-7
2006309 Cortisol, Urine Interpretation 48767-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Cortisol, Free, LC/MS/MS, Second Void Urine
  • Urinary Free Cortisol
Cortisol Urine Free by LC-MS/MS