Ordering Recommendation

Rule-out Cushing syndrome.

Mnemonic
CORT UF
Methodology

Quantitative Liquid Chromatography-Tandem Mass Spectrometry

Performed

Sun-Sat

Reported

1-4 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

24-hour or random urine. Refrigerate 24-hour specimen during collection.

Specimen Preparation

Transport one 4 mL aliquot of urine. (Min: 1 mL) Record total volume and collection time interval on transport tube and test request form.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Room temperature specimens. Acidified specimens or specimens with preservatives.

Remarks
Stability

Ambient: Unacceptable; Refrigerated: 2 weeks; Frozen: 6 months

Reference Interval

Effective April 7, 2014

Test Name
Female
Male
Cortisol, Urine Free - per 24h 3-8 years: Less than or equal to 18 µg/24h
9-12 years: Less than or equal to 37 µg/24h
13-17 years: Less than or equal to 56 µg/24h
18 years and older: Less than or equal to 45 µg/24h
3-8 years: Less than or equal to 18 µg/24h
9-12 years: Less than or equal to 37 µg/24h
13-17 years: Less than or equal to 56 µg/24h
18 years and older: Less than or equal to 60 µg/24h
Cortisol, Urine Free - ratio to CRT Prepubertal: Less than 25 µg/g CRT
18 years and older: Less than 24 µg/g CRT
Pregnancy: Less than 59 µg/g CRT
Prepubertal: Less than 25 µg/g CRT
18 years and older: Less than 32 µg/g CRT

Interpretive Data

Interpretive Data: The optimal specimen for this testing is a 24-hour urine collection. Mass per day calculations are not reported for the following specimen types: a random collection, a collection with duration of less than 20 hours, a collection with duration of greater than 28 hours, or a collection with total volume less than 400 mL or greater than 5000 mL. Ratios to creatinine may be useful for these evaluations.

Baseline urinary free cortisol excretion less than 5 ug/d may be consistent with adrenal insufficiency.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note

Reference intervals based on literature from Taylor R.L. et al., Validation of a High-Throughput Liquid Chromatography-Tandem Mass Spectrometry Method for Urine Cortisol and Cortisone. Clinical Chemistry 2002; 48:1511-1519.

Hotline History
N/A
CPT Codes

82530

Components
Component Test Code* Component Chart Name LOINC
0020207 Creatinine, Urine - per volume 2161-8
0020208 Creatinine, Urine - per 24h 2162-6
0092087 Cortisol, Urine Free - per volume 34909-2
0093444 Cortisol, Urine Free - ratio to CRT 32009-3
0097110 Total Volume 19153-6
0097111 Hours Collected 30211-7
0097224 Cortisol, Urine Free - per 24h 2147-7
2006309 Cortisol, Urine Interpretation 48767-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Cortisol, Free, LC/MS/MS, Second Void Urine
  • Urinary Free Cortisol
Cortisol Urine Free by LC-MS/MS