Ordering Recommendation

Rule-out Cushing syndrome.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

24-hour or random urine. Refrigerate 24-hour specimen during collection.

Specimen Preparation

Transport one 4 mL aliquot of urine. (Min: 1 mL) Record total volume and collection time interval on transport tube and test request form.

Storage/Transport Temperature


Unacceptable Conditions

Room temperature specimens. Acidified specimens or specimens with preservatives.


Ambient: Unacceptable; Refrigerated: 2 weeks; Frozen: 6 months


Quantitative Liquid Chromatography-Tandem Mass Spectrometry




1-5 days

Reference Interval

Test Number
Reference Interval
  Creatinine, Urine - per 24h
Male (mg/d)
Female (mg/d)
3-8 years 140-700 140-700
9-12 years 300-1300 300-1300
13-17 years 500-2300 400-1600
18-50 years 1000-2500 700-1600
51-80 years 800-2100 500-1400
81 years and older 600-2000 400-1300

  Cortisol, Urine Free - ratio to CRT
Age Male (ug/g CRT) Female (ug/g CRT)
Prepubertal Less than 25 Less than 25
18 years and older Less than 32 Less than 24
Pregnancy Not Applicable Less than 59

  Cortisol, Urine Free - per 24h
Age Male (ug/24 h) Female (ug/24 h)
3-8 years Less than or equal to 18 Less than or equal to 18
9-12 years Less than or equal to 37 Less than or equal to 37
13-17 years Less than or equal to 56 Less than or equal to 56
18 years and older Less than or equal to 60 Less than or equal to 45

Interpretive Data

Per 24h calculations are provided to aid interpretation for collections with a duration of 24 hours and an average daily urine volume. For specimens with notable deviations in collection time or volume, ratios of analytes to a corresponding urine creatinine concentration may assist in result interpretation.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


Reference intervals based on literature from Taylor R.L. et al., Validation of a High-Throughput Liquid Chromatography-Tandem Mass Spectrometry Method for Urine Cortisol and Cortisone. Clinical Chemistry 2002; 48:1511-1519.

Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0020207 Creatinine, Urine - per volume 2161-8
0020208 Creatinine, Urine - per 24h 2162-6
0092087 Cortisol, Urine Free - per volume 34909-2
0093444 Cortisol, Urine Free - ratio to CRT 32009-3
0097110 Total Volume 19153-6
0097111 Hours Collected 30211-7
0097224 Cortisol, Urine Free - per 24h 2147-7
2006309 Cortisol, Urine Interpretation 48767-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Cortisol, Free, LC/MS/MS, Second Void Urine
  • Urinary Free Cortisol
Cortisol Urine Free by LC-MS/MS