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Time Sensitive
Ordering Recommendation

Aid in screening for chronic granulomatous disease.

Mnemonic
DHR
Methodology

Semi-Quantitative Flow Cytometry

Performed

Sun-Sat

Reported

2-3 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient.

Collect

Green (Sodium or Lithium Heparin) (patient) AND Green (Sodium or Lithium Heparin) (control). Patient and control specimens must be collected within 48 hours of test performance.

Specimen Preparation

Transport 3 mL whole blood (patient) AND 3 mL whole blood (control) in original collection tubes. (Min: 1 mL (patient) AND 1 mL (control)) Do not refrigerate or freeze. LIVE NEUTROPHILS REQUIRED.

Storage/Transport Temperature

CRITICAL ROOM TEMPERATURE.

Unacceptable Conditions
Remarks
Stability

Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Ambient 24 hours; Refrigerated: Unacceptable; Frozen Unacceptable

Reference Interval

By report

Interpretive Data

White blood cells are incubated with dihydrorhodamine 123 (DHR) and catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA).  Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the cell is measured by flow cytometry. 

Results are reported as the ratio of the mean channel fluorescence of stimulated cells versus unstimulated cells, which yields a stimulation index (SI).

Compliance Category

Laboratory Developed Test (LDT)

Note

If sample shows abnormal results when stimulated, and no control was sent, test should be resubmitted with control sample to validate the conditions of collection, processing and transport.  For abnormal results, we encourage consultation with the ARUP Immunology Medical Director.

Interpretation comparing the patient results to the client normal control and the laboratory control will be provided by the medical director.

Hotline History
N/A
CPT Codes

86352

Components
Component Test Code* Component Chart Name LOINC
0096657 Neutrophil Oxidative Burst 30903-9
2004851 EER Neutrophil Oxidative Burst 11526-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • CGD Test
  • Chronic Granulomatous Disease Test
  • DHR test
  • Neutrophil Oxidation
Neutrophil Oxidative Burst Assay (DHR)