Aid in screening for chronic granulomatous disease.
Semi-Quantitative Flow Cytometry
Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient.
Green (Sodium or Lithium Heparin) (patient) AND Green (Sodium or Lithium Heparin) (control). Patient and control specimens must be collected within 48 hours of test performance.
Transport 3 mL whole blood (patient) AND 3 mL whole blood (control) in original collection tubes. (Min: 1 mL (patient) AND 1 mL (control)) Do not refrigerate or freeze. LIVE NEUTROPHILS REQUIRED.
CRITICAL ROOM TEMPERATURE.
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Ambient 24 hours; Refrigerated: Unacceptable; Frozen Unacceptable
White blood cells are incubated with dihydrorhodamine 123 (DHR) and catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the cell is measured by flow cytometry.
Results are reported as the ratio of the mean channel fluorescence of stimulated cells versus unstimulated cells, which yields a stimulation index (SI).
Laboratory Developed Test (LDT)
If sample shows abnormal results when stimulated, and no control was sent, test should be resubmitted with control sample to validate the conditions of collection, processing and transport. For abnormal results, we encourage consultation with the ARUP Immunology Medical Director.
Interpretation comparing the patient results to the client normal control and the laboratory control will be provided by the medical director.
|Component Test Code*||Component Chart Name||LOINC|
|0096657||Neutrophil Oxidative Burst||30903-9|
|2004851||EER Neutrophil Oxidative Burst||11526-1|
- CGD Test
- Chronic Granulomatous Disease Test
- DHR test
- Neutrophil Oxidation