Time Sensitive

Ordering Recommendation

Monitor response to CD3 immunosuppressive therapy.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Draw specimen before administering immunosuppressive medications.


Green (Sodium or Lithium Heparin), Lavender (EDTA), or Pink (K2EDTA).

Specimen Preparation

Transport 5 mL whole blood. (Min: 0.5 mL)

Storage/Transport Temperature

Room temperature. Also acceptable: Refrigerated.

Unacceptable Conditions

Clotted or hemolyzed specimens.


Specimens must be analyzed within 72 hours of collection.
New York State Clients: Same as the above.


Ambient: 72 hours; Refrigerated: 72 hours; Frozen: Unacceptable
New York State Clients: Same as the above.


Quantitative Flow Cytometry




1-2 days

Reference Interval

Test Number
Reference Interval
  % CD3+/TCR ab+
Reference Interval (%)
17 years and older 56 - 85

  Absolute CD3+/TCR ab+
Age Reference Interval (cells/µL)
17 years or older 633-2532

Interpretive Data

In this test, the percent CD3 cells are those cells that are positive for both CD3 and the alpha-beta chain of the T-cell receptor (TCR) and are presumed immunologically competent.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


THIS TEST IS FOR TRANSPLANT PATIENTS ONLY.  Effective immunosuppressive therapy with OKT3 can be documented by verifying CD3 antigen removal using this test.  For immunocompromised patients, order Lymphocyte Subset Panel 4 - T-Cell subsets Percent & Absolute (0095950).

Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0095248 % CD3+/TCR ab+ 8124-0
0095249 Absolute CD3+/TCR ab+ 8122-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • CD3 absolute
  • immunosuppressive therapy monitoring
Lymphocyte Transplantation CD3