Icon

Time Sensitive
Ordering Recommendation

Monitor response to CD3 immunosuppressive therapy.

Mnemonic
CD3
Methodology

Quantitative Flow Cytometry

Performed

Sun-Sat

Reported

1-2 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Draw specimen before administering immunosuppressive medications.

Collect

Green (Sodium or Lithium Heparin), Lavender (EDTA), or Pink (K2EDTA).

Specimen Preparation

Transport 5 mL whole blood. (Min: 0.5 mL)

Storage/Transport Temperature

Room temperature. Also acceptable: Refrigerated.

Unacceptable Conditions

Clotted or hemolyzed specimens.

Remarks

Specimens must be analyzed within 72 hours of collection.
New York State Clients: EDTA specimens must be analyzed within 30 hours of collection. Heparin specimens must be analyzed within 48 hours of collection.

Stability

Ambient: 72 hours; Refrigerated: 72 hours; Frozen: Unacceptable
New York State Clients: EDTA: Ambient: 30 hours; Refrigerated: 30 hours; Frozen: Unacceptable
Heparin: Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable

Reference Interval

Effective February 20, 2018

Components
17 years and older
%CD3 56-85 %
Abs CD3 633-2532 cells/µL

Interpretive Data

In this test, the percent CD3 cells are those cells that are positive for both CD3 and the alpha-beta chain of the T-cell receptor (TCR) and are presumed immunologically competent.

Compliance Category

Analyte Specific Reagent (ASR)

Note

THIS TEST IS FOR TRANSPLANT PATIENTS ONLY.  Effective immunosuppressive therapy with OKT3 can be documented by verifying CD3 antigen removal using this test.  For immunocompromised patients, order Lymphocyte Subset Panel 4 - T-Cell subsets Percent & Absolute (0095950).

Hotline History
N/A
CPT Codes

86356

Components
Component Test Code* Component Chart Name LOINC
0095248 % CD3+/TCR ab+ 8124-0
0095249 Absolute CD3+/TCR ab+ 8122-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • CD3 absolute
  • immunosuppresive therapy monitoring
Lymphocyte Transplantation CD3