HLA-B27

0095840
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Example Reports
Indeterminate
Negative
Positive

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Time Sensitive

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Additional Technical Information

Ordering Recommendation

Not a diagnostic test for ankylosing spondylitis, juvenile rheumatoid arthritis, or Reiter syndrome. May assist in the diagnosis of these conditions only if other clinical signs and symptoms are present.

Mnemonic

HLAB27

Methodology

Qualitative Flow Cytometry

Performed

Sun-Sat

Reported

1-2 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Lavender Hemogard (EDTA), pink Hemogard (K2EDTA), or green Hemogard (sodium or lithium heparin). Hemogard tubes are preferred for laboratory safety.

Specimen Preparation

Transport 4 mL whole blood. (Min: 0.5 mL)

Storage/Transport Temperature

CRITICAL ROOM TEMPERATURE.

Unacceptable Conditions

Frozen or refrigerated specimens. Specimens older than 72 hours. Clotted or hemolyzed specimens.
New York State Clients: Same as the above.

Remarks

Specimens must be analyzed within 72 hours of collection.
New York State Clients: Same as the above.

Stability

Ambient: 72 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Same as the above.

Reference Interval

Negative

Interpretive Data

HLA-B27 is a serologically-defined allele of the human HLA-B locus. The presence of the HLA-B27 antigen is strongly associated with ankylosing spondylitis and related disorders.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Modified FDA

Note

Indeterminate Results: The Anti-HLA-B27 antibody (clone GS145.2) used in the HLA-B27 assay can cross-react with some other HLA-B locus antigens, creating false-positive results for HLA-B27 expression. Therefore, results falling within the established indeterminate zone will include a recommendation of genotype testing to confirm positive HLA-B27 expression. Order Ankylosing Spondylitis HLA-B27 Genotyping, (ARUP test code 0050392).

Hotline History

N/A

CPT Codes

86812

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Components

Component Test Code* Component Chart Name LOINC
0095030 HLA-B27 26028-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Ankylosing Spondylitis
  • HLA Antigen B27
  • HLA-B27 Antigen
HLA-B27