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HLA-B27
Ordering Recommendation
Not a diagnostic test for ankylosing spondylitis, juvenile rheumatoid arthritis, or Reiter syndrome. May assist in the diagnosis of these conditions only if other clinical signs and symptoms are present.
New York DOH Approval Status
Specimen Required
Lavender Hemogard (EDTA), pink Hemogard (K2EDTA), or green Hemogard (sodium or lithium heparin). Hemogard tubes are preferred for laboratory safety.
Transport 4 mL whole blood. (Min: 0.5 mL)
CRITICAL ROOM TEMPERATURE.
Frozen or refrigerated specimens. Specimens older than 72 hours. Clotted or hemolyzed specimens.
New York State Clients: Same as the above.
Specimens must be analyzed within 72 hours of collection.
New York State Clients: Same as the above.
Ambient: 72 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Same as the above.
Methodology
Qualitative Flow Cytometry
Performed
Sun-Sat
Reported
1-2 days
Reference Interval
Negative
Interpretive Data
HLA-B27 is a serologically-defined allele of the human HLA-B locus. The presence of the HLA-B27 antigen is strongly associated with ankylosing spondylitis and related disorders.
Laboratory Developed Test (LDT)
Note
Indeterminate Results: The Anti-HLA-B27 antibody (clone GS145.2) used in the HLA-B27 assay can cross-react with some other HLA-B locus antigens, creating false-positive results for HLA-B27 expression. Therefore, results falling within the established indeterminate zone will include a recommendation of genotype testing to confirm positive HLA-B27 expression. Order Ankylosing Spondylitis HLA-B27 Genotyping (ARUP test code 0050392).
Hotline History
CPT Codes
86812
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 0095030 | HLA-B27 | 26028-1 |
Aliases
- Ankylosing Spondylitis
- HLA-B27 Antigen
- HLA Antigen B27
