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Time Sensitive
Ordering Recommendation

Support the diagnosis of autoimmune thrombocytopenia (AITP).

Mnemonic
PAIG
Methodology

Qualitative Flow Cytometry

Performed

Sun-Sat

Reported

2-3 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Lavender (EDTA) or pink (K2EDTA).

Specimen Preparation

Transport 4 mL whole blood. (Min: 1 mL)

Storage/Transport Temperature

CRITICAL ROOM TEMPERATURE.

Unacceptable Conditions

Clotted, hemolyzed, frozen, or refrigerated specimens. Specimens older than 48 hours.

Remarks

Specimens must be analyzed within 48 hours of collection. Required amount of blood may be dependent on platelet count.

Stability

Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval

IgG: Negative
IgM: Negative

Interpretive Data

Negative (IgG & IgM): No excess antibodies were associated with the patient's platelets. An immune cause of thrombocytopenia is unlikely.
Positive (IgG and/or IgM): An increase in platelet associated immunoglobulin is noted. An immune cause of thrombocytopenia should be considered. However, many conditions can result in an increase in platelet associated antibodies; for example, IgM rheumatoid factor antibodies.
Strong Positive (IgG and/or IgM): A definite increase in platelet associated immunoglobulin is noted and an immune cause of thrombocytopenia should be considered. However, many conditions can result in an increase in platelet associated antibodies; for example, IgM rheumatoid factor antibodies.

Compliance Statement A: For laboratory developed tests using a manufacturer labeled ASR as the reagent providing the specificity of the assay. Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

Note

Detection of platelet-associated IgG and/or IgM may be used to separate thrombocytopenia of immune origin from nonimmune origin. Most patients with ITP have abnormally high levels of IgG associated with their platelets. Occasionally patients will have normal IgG levels but abnormally high levels of IgM. Dual staining and flow cytometric analysis ensures that only platelets are analyzed and relatively small volumes of blood are required. This assay does not distinguish between autoantibodies and alloantibodies, nor does it identify specific types of antiplatelet antibodies, such as those against HPA-1a. Refer to Platelet Antibodies, Indirect (ARUP test code 0051050).

Hotline History
N/A
CPT Codes

86023 x2

Components
Component Test Code* Component Chart Name LOINC
0095612 Platelet Antibodies Direct, IgG 29497-5
0095613 Platelet Antibodies Direct, IgM 29498-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Direct Platelet Antibody
  • Anti-Platelet Antibodies
  • Cell-Bound Platelet Antibody Test
  • Platelet Associated Immunoglobulins (IgG & IgM)
Platelet Associated Antibodies, Direct Assay