Lymphocyte Subset Panel 4 - T-Cell Subsets Percent and Ratio, Bronchoalveolar Lavage
Use to support a diagnosis of sarcoidosis.
New York DOH Approval Status
Bronchoalveolar lavage (Bronch Wash or BAL).
Transfer 4 mL bronchoalveolar lavage to a sterile container. (Min: 3 mL)
Whole blood (refer to ARUP test code 0095950). Excess mucus, excess peripheral blood, or too few lymphocytes. Frozen or room temperature specimens. Specimens older than 48 hours. Contaminated specimens.
Specimens must be analyzed within 48 hours of collection.
Separate orders must be submitted for multiple samples from different source sites. The collection time may need to vary in submitting samples with the same collection date. An except will be created if multiple samples are submitted under one order only.
Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: Unacceptable
A CD4/CD8 ratio greater than 3.5 is suggestive of sarcoidosis; If the CD4/CD8 ratio is greater than 5.0 and clinical and radiographic findings are compatible with sarcoidosis, some institutions accept a diagnosis of sarcoidosis even with a negative biopsy; In extrinsic allergic alveolitis (EAA), the CD4/CD8 ratio is decreased due to a relative increase in the CD8 positive T-cells.
Laboratory Developed Test (LDT)
This test is designed for enumerating the percent of T-cell lymphocyte subsets in BAL. Cells from the BAL are added to fluorochrome-labeled antibodies that bind specifically to cell surface antigens on lymphocytes. The CD4/CD8 ratio is calculated by dividing the CD4 percent by the CD8 percent.
|Component Test Code*||Component Chart Name||LOINC|
|0093421||% CD3 - BAL||97594-6|
|0093422||% CD4 - BAL||97595-3|
|0093423||% CD8 - BAL||97596-1|
|0093424||CD4:CD8 Ratio - BAL||97599-5|
- BAL for CD4/CD8 Ratio
- Bronch Wash for CD4:CD8 Ratio