Ordering Recommendation

Recommended test for evaluating endogenous estrogen status in postmenopausal women, men, or children.

Mnemonic

EST FR TMS

Methodology

Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry

Performed

Sun-Sat

Reported

1-4 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube, lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation

Separate serum or plasma from cells within 2 hours of collection. Transfer 0.5 mL serum or plasma to an ARUP Standard Transport Tube. (Min 0.3 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions
Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 1 week; Frozen: 1 month

Reference Interval

Test Number
Components
Reference Interval
0093247 Estradiol, Males, Children or Postmenopausal Females by Tandem Mass Spectrometry
Reference Intervals for Estradiol-Children
Tanner Stages
I
II
III
IV and V
Males
less than 8.0 pg/mL
less than 10.0 pg/mL
1.0-35.0 pg/mL
3.0-35.0 pg/mL
Females
less than 56.0 pg/mL
2.0-133.0 pg/mL
12.0-277.0 pg/mL
2.0-259.0 pg/mL
Age Group
7-9
10-12
13-15
16-17
Males
less than 7.0 pg/mL
less than 11.0 pg/mL
1.0-36.0 pg/mL
3.0-34.0 pg/mL
Females
less than 36.0 pg/mL
1.0-87.0 pg/mL
9.0-249.0 pg/mL
2.0-266.0 pg/mL

Reference Intervals for Estradiol-Adults
Females 18 Years and Older
Males 18 years and Older
Pre-menopausal:
Early Follicular: 30.0-100.0 pg/mL
Late Follicular: 100.0-400.0 pg/mL
Luteal: 50.0-150.0 pg/mL
10.0-42.0 pg/mL
Postmenopausal: 2.0-21.0 pg/mL

0093249 Estrone, by Tandem Mass Spectrometry
Reference Intervals for Estrone-Children
Tanner Stages:
I
II
III
IV and V
Males
less than 7.0 pg/mL
less than 11.0 pg/mL
1.0-31.0 pg/mL
2.0-30.0 pg/mL
Females
less than 27.0 pg/mL
1.0-39.0 pg/mL
8.0-117.0 pg/mL
4.0-109.0 pg/mL
Age Group
7-9
10-12
13-15
16-17
Males
less than 7.0 pg/mL
less than 11.0 pg/mL
1.0-30.0 pg/mL
1.0-32.0 pg/mL
Females
less than 20.0 pg/mL
1.0-40.0 pg/mL
8.0-105.0 pg/mL
4.0-133.0 pg/mL

Reference Intervals for Estrone-Adults
Females 18 Years and Older
Males 18 Years and Older
Premenopausal:
Early Follicular: less than 150.0 pg/mL
Late Follicular: 100.0-250.0 pg/mL
Luteal: less than 200.0 pg/mL
9.0-36.0 pg/mL
Postmenopausal: 3.0-32.0 pg/mL

  Estrogens Total Calculation
Reference Intervals of Estrogens
Tanner Stages:
I
II
III
IV and V
Males
1.0-11.0
1.0-19.0
3.0-61.0
4.0-62.0
Females
1.0-86.0
3.0-169.0
23.0-351.0
8.0-341.0
Age Group:
7-9
10-12
13-15
16-17
Males
less than 10.0
1.0-19.0
3.0-62.0
4.0-64.0
Females
1.0-48.0
2.0-116.0
15.0-333.0
6.0-354.0

Reference Intervals of Estrogens of Adults
Females 18 Years or Older
Males 18 Years or Older
Total Estrogens (estrone+ estradiol) pg/mL
Total Estrogens (estrone+ estradiol) pg/mL
Pre-menopausal:
Early Follicular: 30.0-250.0
Late Follicular: 200.0-650.0
Luteal: 50.0-350.0
19.0-69.0
Post-menopausal: 5.0-52.0

Interpretive Data

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note

Hotline History

N/A

CPT Codes

82671

Components

Component Test Code* Component Chart Name LOINC
0093247 Estradiol by TMS 35384-7
0093249 Estrone by TMS 2258-2
0093251 Estrogens Total Calculation 53765-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Estrone (E1) and Estradiols (E2)
  • Total Estrogens
Estrogens, Fractionated by Tandem Mass Spectrometry