Ordering Recommendation

Use to evaluate estrogen status in children, cisgender males, and postmenopausal cisgender females. Most useful when low estrogen concentrations are expected, regardless of the patient’s sex assigned at birth.

To compare this test to other estrogen tests, refer to the ARUP Estrogen Tests Comparison table.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum separator tube, lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation

Separate serum or plasma from cells within 2 hours of collection. Transfer 0.5 mL serum or plasma to an ARUP Standard Transport Tube. (Min 0.3 mL)

Storage/Transport Temperature


Unacceptable Conditions

After separation from cells: Ambient: 48 hours; Refrigerated: 1 week; Frozen: 1 month


Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry




1-5 days

Reference Interval

Test Number
Reference Interval
  Estradiol by Mass Spec
Male (pg/mL)
Female (pg/mL)
7-9 years Less than 7.0 Less than 36.0
10-12 years Less than 11.0 1.0-87.0
13-15 years 1.0-36.0 9.0-249.0
16-17 years 3.0-34.0 2.0-266.0
18 years and older 10.0-42.0 Premenopausal
Early Follicular: 30.0-100.0
Late Follicular: 100.0-400.0
Luteal: 50.0-150.0

Postmenopausal: 2.0-21.0
Tanner Stage I Less than 8.0 Less than 56.0
Tanner Stage II Less than 10.0 2.0-133.0
Tanner Stage III 1.0-35.0 12.0-277.0
Tanner Stage IV-V 3.0-35.0 2.0-259.0

  Estrone by Mass Spec
Age Male (pg/mL) Female (pg/mL)
7-9 years Less than 7.0 Less than 20.0
10-12 years Less than 11.0 1.0-40.0
13-15 years 1.0-30.0 8.0-105.0
16-17 years 1.0-32.0 4.0-133.0
18 years and older 9.0-36.0 Premenopausal
Early Follicular: Less than 150.0
Late Follicular: 100.0-250.0.
Luteal: Less than 200.0

Postmenopausal: 3.0-32.0
Tanner Stage I Less than 7.0 Less than 27.0
Tanner Stage II Less than 11.0 1.0-39.0
Tanner Stage III 1.0-31.0 8.0-117.0
Tanner Stage IV-V 2.0-30.0 4.0-109.0

  Estrogens Total Calculation
Age Male (pg/mL) Female (pg/mL)
7-9  years Less than 10.0 1.0-48.0
10-12 years 1.0-19.0 2.0-116.0
13-15 years 3.0-62.0 15.0-333.0
16-17 years 4.0-64.0 6.0-354.0
18 years or older 19.0-69.0 Premenopausal
Early Follicular: 30.0-250.0
Late Follicular: 200.0-650.0
Luteal: 50.0-350.0

Postmenopausal: 5.0-52.0
Tanner Stage I 1.0-11.0 1.0-86.0
Tanner Stage II 1.0-19.0 3.0-169.0
Tanner Stage III 3.0-61.0 23.0-351.0
Tanner Stage IV-V 4.0-62.0 8.0-341.0

Interpretive Data

For a complete set of all established reference intervals, refer to ltd.aruplab.com/Tests/Pub/0093248.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0093247 Estradiol by Mass Spec 35384-7
0093249 Estrone by Mass Spec 2258-2
0093251 Estrogens Total Calculation 53765-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Estrone (E1) and Estradiols (E2)
  • Total Estrogens
Estrogens, Fractionated, by Mass Spectrometry