Ordering Recommendation

Use this mass spectrometry test to measure a wide range of estradiol concentrations. Most useful when low estradiol concentrations are expected, regardless of the patient’s sex assigned at birth. Use to monitor estradiol in individuals on estrogen-suppressing hormone therapies (eg, aromatase inhibitors or testosterone).

To compare this test to other estrogen tests, refer to the ARUP Estrogen Tests Comparison table.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum separator tube, lavender (EDTA), pink (K2EDTA) or green (sodium or lithium heparin).

Specimen Preparation

Separate serum or plasma from cells within 2 hours after collection. Transfer 0.5 mL serum or plasma to an ARUP Standard Transport Tube. (Min 0.3 mL) Indicate age and sex of patient on test request form AND specimen tube.

Storage/Transport Temperature


Unacceptable Conditions

After separation from cells: Ambient: 48 hours; Refrigerated: 1 week; Frozen: 1 month


Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry




1-5 days

Reference Interval

Test Number
Reference Interval
  Estradiol by Mass Spec
Male (pg/mL)
Female (pg/mL)
7-9 years Less than 7.0 Less than 36.0
10-12 years Less than 11.0 1.0-87.0
13-15 years 1.0-36.0 9.0-249.0
16-17 years 3.0-34.0 2.0-266.0
18 years and older 10.0-42.0 Premenopausal
Early Follicular: 30.0-100.0
Late Follicular: 100.0-400.0
Luteal: 50.0-150.0

Postmenopausal: 2.0-21.0
Tanner Stage I Less than 8.0 Less than 56.0
Tanner Stage II Less than 10.0 2.0-133.0
Tanner Stage III 1.0-35.0 12.0-277.0
Tanner Stage IV-V 3.0-35.0 2.0-259.0

Interpretive Data

For a complete set of all established reference intervals, refer to ltd.aruplab.com/Tests/Pub/0093247.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0093247 Estradiol by Mass Spec 35384-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Estradiol (E2)
  • Ultrasensitive Estradiol
Estradiol (Adult Males, Children, Postmenopausal Females, or Individuals on Estrogen-Suppressing Hormone Therapy)