Evaluate NK cells in patients with suspected immune deficiency.
Quantitative Flow Cytometry
Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin). Hemogard tubes are preferred for laboratory automation and safety.
Transport 4 mL whole blood. (Min: 0.5 mL)
CRITICAL ROOM TEMPERATURE.
Clotted or hemolyzed specimens.
Specimens must be analyzed within stability times provided. Some medication may affect immunophenotyping results and should be provided on the patient test request form.
EDTA: Ambient: 72 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Heparin: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Same as the above.
Effective November 16, 2015
Reference Interval Notes:
Pediatric reference values (0 - 6 days up to 10 - 15 years) taken from Scandinavian Journal of Immunology 2012; 75, 436-444.
Adult and Geriatric (16 - 64 and 65 plus years) ranges were developed in-lab.
||Components||0-6 days||1 week - 1 month||2-4 months||5-8 months||9-14 months||15-23 months||2-4 years||5-9 years||10-15 years||16-64 years||65 years or older|
|Absolute NK-cells||500-3100 cells/µL||140-1900 cells/µL||97-2000 cells/µL||68-3900 cells/µL||71-3500 cells/µL||55-4000 cells/µL||61-510 cells/µL||70-590 cells/µL||92-1200 cells/µL||78-470 cells/µL||74-620 cells/µL|
The results reported are the percentage of lymphocytes that are Natural killer (NK) cells and the absolute count of NK-cells per µL of blood. Only those cells that express CD45 and either CD16 or CD56 (or both) but not CD3 or CD19 are reported.
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Laboratory Developed Test (LDT)
|Component Test Code*||Component Chart Name||LOINC|
|0092403||Natural Killer Cell Information|
|0095701||% Natural Killer Cells||32519-1|
|0095702||Absolute Natural Killer Cells||20604-5|
- Natural Killer Cell CD56/CD16, Percent and Absolute
- NK absolute cell count and percentage