Natural Killer Cells Enumeration

0092404
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Abnormal
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Time Sensitive
Ordering Recommendation

Evaluate NK cells in patients with suspected immune deficiency.

Mnemonic
NK CELLS
Methodology

Quantitative Flow Cytometry

Performed

Sun-Sat

Reported

1-3 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin). Hemogard tubes are preferred for laboratory automation and safety.

Specimen Preparation

Transport 4 mL whole blood. (Min: 0.5 mL)

Storage/Transport Temperature

CRITICAL ROOM TEMPERATURE.

Unacceptable Conditions

Clotted or hemolyzed specimens.

Remarks

Specimens must be analyzed within stability times provided. Some medication may affect immunophenotyping results and should be provided on the patient test request form.

Stability

EDTA: Ambient: 72 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Heparin: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: EDTA: Ambient: 30 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Heparin: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval

Effective November 16, 2015

Reference Interval Notes:
Pediatric reference values (0 - 6 days up to 10 - 15 years) taken from Scandinavian Journal of Immunology 2012; 75, 436-444.
Adult and Geriatric (16 - 64 and 65 plus years) ranges were developed in-lab.

Test Number
 Components 0-6 days 1 week - 1 month 2-4 months 5-8 months 9-14 months 15-23 months 2-4 years 5-9 years 10-15 years 16-64 years 65 years or older
% NK-cells 8-62% 3-23% 2-20% 2-36% 1-64% 1-96% 2-25% 2-31% 4-51% 4-26% 5-28%
Absolute NK-cells 500-3100 cells/µL 140-1900 cells/µL 97-2000 cells/µL 68-3900 cells/µL 71-3500 cells/µL 55-4000 cells/µL 61-510 cells/µL 70-590 cells/µL 92-1200 cells/µL 78-470 cells/µL 74-620 cells/µL

Interpretive Data

The results reported are the percentage of lymphocytes that are Natural killer (NK) cells and the absolute count of NK-cells per µL of blood. Only those cells that express CD45 and either CD16 or CD56 (or both) but not CD3 or CD19 are reported.

Compliance Category

Laboratory Developed Test (LDT)

Note
Hotline History
N/A
CPT Codes

86357

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Components
Component Test Code* Component Chart Name LOINC
0092403 Natural Killer Cell Information
0095701 % Natural Killer Cells 32519-1
0095702 Absolute Natural Killer Cells 20604-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Natural Killer Cell CD56/CD16, Percent and Absolute
  • NK absolute cell count and percentage
Natural Killer Cells Enumeration