Ordering Recommendation

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum separator tube. Also acceptable: Plain red, lavender (EDTA), pink  (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum or plasma to an ARUP Standard Transport Tube and freeze immediately. (Min: 0.25 mL)

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions

Refrigerated or room temperature specimens.


After separation from cells: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 6 months


Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry


Mon, Fri


1-5 days

Reference Interval

Test Number
Reference Interval
  17-Hydroxypregnenolone 60-Minute
Age Male (ng/dL) Female (ng/dL)
1-5 months 633-3286 633-3286
6-11 months 257-2173 257-2173
1-5 years 45-740 45-740
6-12 years 70-660 70-660
Early puberty 88-675 251-756
Late puberty 220-966 502-1402
Adult 240-1000 290-1382
Tanner Stage II-III 88-675 250-800
Tanner Stage IV-V 220-860 500-1600

Interpretive Data

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0092337 17-Hydroxypregnenolone 60-Minute 49325-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


17-Hydroxypregnenolone 60-Minute Timed Specimen