Ordering Recommendation

Detect accumulation of specific steroids as a result of enzyme deficiencies in congenital adrenal hyperplasia (CAH). Monitor patients with CAH.

Mnemonic
ADSTEROID
Methodology

Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry

Performed

Mon-Fri

Reported

1-4 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube. Also acceptable: Plain red, pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1.2 mL serum or plasma to an ARUP Standard Transport Tube and freeze immediately. (Min: 0.6 mL)

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions

Refrigerated or room temperature specimens.

Remarks
Stability

After separation from cells: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 6 months

Reference Interval
Test Number
Components
Reference Interval
0092331 11-Deoxycortisol Quantitative by HPLC-MS/MS, Serum or Plasma Effective August 19, 2013
Age
Female
Male
Premature (26-28 weeks) 110-1376 ng/dL 110-1376 ng/dL
Premature (29-36 weeks) 70-455 ng/dL 70-455 ng/dL
Full Term (1-5 months) 10-200 ng/dL 10-200 ng/dL
6-11 months 10-276 ng/dL 10-276 ng/dL
1-3 years 7-247 ng/dL 7-202 ng/dL
4-6 years 8-291 ng/dL 8-235 ng/dL
7-9 years Less than or equal to 94 ng/dL Less than or equal to 120 ng/dL
10-12 years Less than or equal to 123 ng/dL Less than or equal to 92 ng/dL
13-15 years Less than or equal to 107 ng/dL Less than or equal to 95 ng/dL
16-17 years Less than or equal to 47 ng/dL Less than or equal to 106 ng/dL
18 years and older Less than 33 ng/dL Less than 50 ng/dL
Tanner Stage I Less than or equal to 94 ng/dL Less than or equal to 105 ng/dL
Tanner Stage II Less than or equal to 136 ng/dL Less than or equal to 108 ng/dL
Tanner Stage III Less than or equal to 99 ng/dL Less than or equal to 111 ng/dL
Tanner Stage IV & V Less than or equal to 50 ng/dL Less than or equal to 83 ng/dL
After metyrapone stimulation Greater than 8000 ng/dL Greater than 8000 ng/dL

0092332 17-Hydroxyprogesterone Quantitative by HPLC-MS/MS, Serum or Plasma Effective August 19, 2013
Age
Female
Male
Premature (26-28 weeks) 124-841 ng/dL 124-841 ng/dL
Premature (29-35 weeks) 26-568 ng/dL 26-568 ng/dL
Full term Day 3 7-77 ng/dL 7-77 ng/dL
4 days-30 days 7-106 ng/dL Less than 200 ng/dL
1 month-2 months 13-106 ng/dL Less than 200 ng/dL
3 months-5 months 13-106 ng/dL 3-90 ng/dL
6  months-1 year Less than or equal to 148 ng/dL Less than or equal to 148 ng/dL
2-3 years Less than or equal to 256 ng/dL Less than or equal to 228 ng/dL
4-6 years Less than or equal to 299 ng/dL Less than or equal to 208 ng/dL
7-9 years Less than or equal to 71 ng/dL Less than or equal to 63 ng/dL
10-12 years Less than or equal to 129 ng/dL Less than or equal to 79 ng/dL
13-15 years 9-208 ng/dL 9-140 ng/dL
16-17 years Less than or equal to 178 ng/dL 24-192 ng/dL
18 years and older Less than 207 ng/dL Less than 139 ng/dL
Follicular 15-70 ng/dL Does Not Apply
Luteal 35-290 ng/dL Does Not Apply
Tanner Stage I Less than or equal to 74 ng/dL Less than or equal to 62 ng/dL
Tanner Stage II Less than or equal to 164 ng/dL Less than or equal to 104 ng/dL
Tanner Stage III 13-209 ng/dL Less than or equal to 151 ng/dL
Tanner Stage IV-V 7-170 ng/dL 20-173 ng/dL

0092333 17-Hydroxypregnenolone Quantitative by LC-MS/MS, Serum or Plasma
Age
Female
Male
Premature (26-28 weeks) 1219-9799 ng/dL 1219-9799 ng/dL
Premature (29-36 weeks) 346-8911 ng/dL 346-8911 ng/dL
Full Term (1-5 months) 229-3104 ng/dL 229-3104 ng/dL
6-12 months less than or equal to 917ng/dL less than or equal to 917ng/dL
13-23 months less than or equal to 592 ng/dL less than or equal to 592 ng/dL
2-4 years less than or equal to 280 ng/dL less than or equal to 249 ng/dL
5-6 years less than or equal to 350 ng/dL less than or equal to 319 ng/dL
7-9 years less than or equal to 212 ng/dL less than or equal to 187 ng/dL
10-12 years less than or equal to 398 ng/dL less than or equal to 392 ng/dL
13-15 years less than or equal to 407 ng/dL 35-465 ng/dL
16-17 years less than or equal to 423 ng/dL 32-478 ng/dL
18 years and older Less than 226 ng/dL Less than 442 ng/dL
Tanner Stage I less than or equal to 235 ng/dL less than or equal to 208 ng/dL
Tanner Stage II less than or equal to 367 ng/dL less than or equal to 355 ng/dL
Tanner Stage III less than or equal to 430 ng/dL less than or equal to 450 ng/dL
Tanner Stage IV-V less than or equal to 412 ng/dL 35-478 ng/dL

0092334 Pregnenolone by LC-MS/MS, Serum or Plasma
Age
Female
Male
6-12 months 13-327 ng/dL 13-327 ng/dL
13-23 months 12-171 ng/dL 12-171 ng/dL
2-4 years 15-125 ng/dL 10-125 ng/dL
5-6 years 13-191 ng/dL 10-156 ng/dL
7-9 years 14-150 ng/dL 13-205 ng/dL
10-12 years 19-220 ng/dL 15-151 ng/dL
13-15 years 22-210 ng/dL 18-197 ng/dL
16-17 years 22-229 ng/dL 17-228 ng/dL
18 years and older 15-132 ng/dL 23-173 ng/dL
Tanner Stage I 15-171 ng/dL 13-156 ng/dL
Tanner Stage II 22-229 ng/dL 12-143 ng/dL
Tanner Stage III 34-215 ng/dL 16-214 ng/dL
Tanner Stage IV-V 26-235 ng/dL 19-201 ng/dL

Interpretive Data



Compliance Category

Laboratory Developed Test (LDT)

Note
Hotline History
N/A
CPT Codes

82634; 83498; 84143; 84140

Components
Component Test Code* Component Chart Name LOINC
0092331 11-Deoxycortisol, HPLC-MS/MS 1657-6
0092332 17-Hydroxyprogesterone, HPLC-MS/MS 1668-3
0092333 17-Hydroxypregnenolone Quant, MS/MS, Ser 6765-2
0092334 Pregnenolone 2837-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • 11-Deoxycortisol
  • 17-Hydroxypregnenolone
  • 17-Hydroxyprogesterone
  • CAH
  • Congenital Adrenal Hyperplasia
  • Pregnenolone
Adrenal Steroid Quantitative Panel by HPLC-MS/MS, Serum or Plasma