Ordering Recommendation

Detect accumulation of specific steroids as a result of enzyme deficiencies in congenital adrenal hyperplasia (CAH). Monitor patients with CAH.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube. Also acceptable: Plain red, pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1.2 mL serum or plasma to an ARUP standard transport tube and freeze immediately. (Min: 0.6 mL)

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions

Refrigerated or room temperature specimens.

Remarks
Stability

After separation from cells: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 6 months

Methodology

Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry

Performed

Mon-Fri

Reported

1-5 days

Reference Interval

Test Number
Components
Reference Interval
  11-Deoxycortisol, HPLC-MS/MS
Age
Male (ng/dL)
Female (ng/dL)
Premature (26-28 weeks) 110-1376 110-1376
Premature (29-36 weeks) 70-455 70-455
Full Term (1-5 months) 10-200 10-200
6-11 months 10-276 10-276
1-3 years 7-202 7-247
4-6 years 8-235 8-291
7-9 years Less than or equal to 120 Less than or equal to 94
10-12 years Less than or equal to 92 Less than or equal to 123
13-15 years Less than or equal to 95 Less than or equal to 107
16-17 years Less than or equal to 106 Less than or equal to 47
18 years and older Less than 50 Less than 33
Tanner Stage I Less than or equal to 105 Less than or equal to 94
Tanner Stage II Less than or equal to 108 Less than or equal to 136
Tanner Stage III Less than or equal to 111 Less than or equal to 99
Tanner Stage IV & V Less than or equal to 83 Less than or equal to 50
After metyrapone stimulation Greater than 8000 Greater than 8000

  17-Hydroxyprogesterone, HPLC-MS/MS
Age
Male (ng/dL)
Female (ng/dL)
Premature (26-28 weeks) 124-841 124-841
Premature (29-35 weeks) 26-568 26-568
Full term Day 3 7-77 7-77
4 days-30 days Less than 200 7-106
1 month-2 months Less than 200 13-106
3 months-5 months 3-90 13-106
6 months-1 year Less than or equal to 148 Less than or equal to 148
2-3 years Less than or equal to 228 Less than or equal to 256
4-6 years Less than or equal to 208 Less than or equal to 299
7-9 years Less than or equal to 63 Less than or equal to 71
10-12 years Less than or equal to 79 Less than or equal to 129
13-15 years 9-140 9-208
16-17 years 24-192 Less than or equal to 178
18 years and older Less than 139 Less than 207
Follicular Not Applicable 15-70
Luteal Not Applicable 35-290
Tanner Stage I Less than or equal to 62 Less than or equal to 74
Tanner Stage II Less than or equal to 104 Less than or equal to 164
Tanner Stage III Less than or equal to 151 13-209
Tanner Stage IV-V 20-173 7-170

  17-Hydroxypregnenolone Quant, MS/MS, Ser
Age Male (ng/dL) Female (ng/dL)
Premature (26-28 weeks) 1219-9799 1219-9799
Premature (29-36 weeks) 346-8911 346-8911
Full Term (1-5 months) 229-3104 229-3104
6-12 months Less than or equal to 917 Less than or equal to 917
13-23 months Less than or equal to 592 Less than or equal to 592
2-4 years Less than or equal to 249 Less than or equal to 280
5-6 years Less than or equal to 319 Less than or equal to 350
7-9 years Less than or equal to 187 Less than or equal to 212
10-12 years Less than or equal to 392 Less than or equal to 398
13-15 years 35-465 Less than or equal to 407
16-17 years 32-478 Less than or equal to 423
18 years and older Less than 442 Less than 226
Tanner Stage I Less than or equal to 208 Less than or equal to 235
Tanner Stage II Less than or equal to 355 Less than or equal to 367
Tanner Stage III Less than or equal to 450 Less than or equal to 430
Tanner Stage IV & V 35-478 Less than or equal to 412

  Pregnenolone
Age Male (ng/dL) Female (ng/dL)
6-12 months 13-327 13-327
13-23 months 12-171 12-171
2-4 years 10-125 15-125
5-6 years 10-156 13-191
7-9 years 13-205 14-150
10-12 years 15-151 19-220
13-15 years 18-197 22-210
16-17 years 17-228 22-229
18 years and older 23-173 15-132
Tanner Stage I 13-156 15-171
Tanner Stage II 12-143 22-229
Tanner Stage III 16-214 34-215
Tanner Stage IV & V 19-201 26-235

Interpretive Data



Compliance Category

Laboratory Developed Test (LDT)

Note

Hotline History

N/A

CPT Codes

82634; 83498; 84143; 84140

Components

Component Test Code* Component Chart Name LOINC
0092331 11-Deoxycortisol, HPLC-MS/MS 1657-6
0092332 17-Hydroxyprogesterone, HPLC-MS/MS 1668-3
0092333 17-Hydroxypregnenolone Quant, MS/MS, Ser 6765-2
0092334 Pregnenolone 2837-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • 11-Deoxycortisol
  • 17-Hydroxypregnenolone
  • 17-Hydroxyprogesterone
  • CAH
  • Congenital Adrenal Hyperplasia
  • Pregnenolone
Adrenal Steroid Quantitative Panel by HPLC-MS/MS, Serum or Plasma