Ordering Recommendation
Mnemonic
Methodology
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
Performed
Sun-Sat
Reported
1-4 days
New York DOH Approval Status
Specimen Required
24-hour or random urine. Refrigerate 24-hour specimen during collection.
Transport one 4 mL aliquot of urine. (Min: 1 mL) Record total volume and collection time interval on transport tube and test request form.
Refrigerated.
Room temperature specimens. Acidified specimens or specimens with preservatives.
Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 1 month
Reference Interval
Effective April 7, 2014
Test Name |
Female |
Male |
---|---|---|
Cortisol, Urine Free - per 24h | 3-8 years: Less than or equal to 18 µg/24h 9-12 years: Less than or equal to 37 µg/24h 13-17 years: Less than or equal to 56 µg/24h 18 years and older: Less than or equal to 45 µg/24h |
3-8 years: Less than or equal to 18 µg/24h 9-12 years: Less than or equal to 37 µg/24h 13-17 years: Less than or equal to 56 µg/24h 18 years and older: Less than or equal to 60 µg/24h |
Cortisol/Cortisone, Ratio* | 0-17 years: To be determined 18 years and older: 0.15-0.50 |
0-17 years: To be determined 18 years and older: 0.15-0.50 |
Cortisone, Urine Free - per volume | Value used to calculate Cortisol/Cortisone ratio | |
Cortisol, Urine Free - ratio to CRT | Prepubertal: Less than 25 µg/g CRT 18 years and older: Less than 24 µg/g CRT Pregnancy: Less than 59 µg/g CRT |
Prepubertal: Less than 25 µg/g CRT 18 years and older: Less than 32 µg/g CRT |
Interpretive Data
Per 24h calculations are provided to aid interpretation for collections with a duration of 24 hours and an average daily urine volume. For specimens with notable deviations in collection time or volume, ratios of analytes to a corresponding urine creatinine concentration may assist in result interpretation.
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Laboratory Developed Test (LDT)
Note
Reference intervals for children are based on literature from Taylor R.L. et al., Validation of a High-Throughput Liquid Chromatography-Tandem Mass Spectrometry Method for Urine Cortisol and Cortisone. Clinical Chemistry 2002; 48:1511-19.
*The ratio of the concentrations of cortisol to cortisone will not be evaluated if the cortisol concentration is less than 5 µg/L.
Hotline History
CPT Codes
82530; 83789
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
0020207 | Creatinine, Urine - per volume | 2161-8 |
0020208 | Creatinine, Urine - per 24h | 2162-6 |
0020581 | Cortisone, Urine Free - per 24h | 14044-2 |
0020582 | Cortisol/Cortisone Ratio | 49029-2 |
0092087 | Cortisol, Urine Free - per volume | 34909-2 |
0092088 | Cortisone, Urine Free - per volume | 30072-3 |
0093443 | Cortisone, Urine Free - ratio to CRT | 30511-0 |
0093444 | Cortisol, Urine Free - ratio to CRT | 32009-3 |
0097110 | Total Volume | 19153-6 |
0097111 | Hours Collected | 30211-7 |
0097224 | Cortisol, Urine Free - per 24h | 2147-7 |
2006310 | Cortisol/Cortisone, Urine Interpretation | 48767-8 |