Chat
Cortisol/Cortisone Urine Free by LC-MS/MS
Copy Utility
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
Sun-Sat
1-4 days
24-hour or random urine. Refrigerate 24-hour specimen during collection.
Transport one 4 mL aliquot of urine. (Min: 1 mL) Record total volume and collection time interval on transport tube and test request form.
Refrigerated.
Room temperature specimens. Acidified specimens or specimens with preservatives.
Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 1 month
Effective April 7, 2014
| Test Name |
Female |
Male |
|---|---|---|
| Cortisol, Urine Free - per 24h | 3-8 years: Less than or equal to 18 µg/24h 9-12 years: Less than or equal to 37 µg/24h 13-17 years: Less than or equal to 56 µg/24h 18 years and older: Less than or equal to 45 µg/24h |
3-8 years: Less than or equal to 18 µg/24h 9-12 years: Less than or equal to 37 µg/24h 13-17 years: Less than or equal to 56 µg/24h 18 years and older: Less than or equal to 60 µg/24h |
| Cortisol/Cortisone, Ratio* | 0-17 years: To be determined 18 years and older: 0.15-0.50 |
0-17 years: To be determined 18 years and older: 0.15-0.50 |
| Cortisone, Urine Free - per volume | Value used to calculate Cortisol/Cortisone ratio | |
| Cortisol, Urine Free - ratio to CRT | Prepubertal: Less than 25 µg/g CRT 18 years and older: Less than 24 µg/g CRT Pregnancy: Less than 59 µg/g CRT |
Prepubertal: Less than 25 µg/g CRT 18 years and older: Less than 32 µg/g CRT |
The optimal specimen for this testing is a 24-hour urine collection. Mass per day calculations are not reported for the following specimen types: a random collection, a collection with duration of less than 20 hours, a collection with duration of greater than 28 hours, or a collection with total volume less than 400 mL or greater than 5000 mL. Ratios to creatinine may be useful for these evaluations.
Baseline urinary free cortisol excretion less than 5 ug/d may be consistent with adrenal insufficiency.
Laboratory Developed Test (LDT)
Reference intervals for children are based on literature from Taylor R.L. et al., Validation of a High-Throughput Liquid Chromatography-Tandem Mass Spectrometry Method for Urine Cortisol and Cortisone. Clinical Chemistry 2002; 48:1511-19.
*The ratio of the concentrations of cortisol to cortisone will not be evaluated if the cortisol concentration is less than 5 µg/L.
82530; 83789
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 0020207 | Creatinine, Urine - per volume | 2161-8 |
| 0020208 | Creatinine, Urine - per 24h | 2162-6 |
| 0020581 | Cortisone, Urine Free - per 24h | 14044-2 |
| 0020582 | Cortisol/Cortisone Ratio | 49029-2 |
| 0092087 | Cortisol, Urine Free - per volume | 34909-2 |
| 0092088 | Cortisone, Urine Free - per volume | 30072-3 |
| 0093443 | Cortisone, Urine Free - ratio to CRT | 30511-0 |
| 0093444 | Cortisol, Urine Free - ratio to CRT | 32009-3 |
| 0097110 | Total Volume | 19153-6 |
| 0097111 | Hours Collected | 30211-7 |
| 0097224 | Cortisol, Urine Free - per 24h | 2147-7 |
| 2006310 | Cortisol/Cortisone, Urine Interpretation | 48767-8 |
Feedback
