Ordering Recommendation

Screening test for tricyclic antidepressants including amitriptyline, clomipramine, desipramine, doxepin, imipramine, nortriptyline, protriptyline, and trimipramine. Results are unconfirmed and are to be used for medical (treatment) purposes only.

Mnemonic
S TAD
Methodology

Enzyme Immunoassay

Performed

Sun-Sat

Reported

Within 24 hours

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Plain red.

Specimen Preparation

Separate serum from cells within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Separator tubes.

Remarks
Stability

After separation from cells: Ambient: 24 hours; Refrigerated: 1 month; Frozen: 1 year

Reference Interval

Negative

Interpretive Data

This test is positive when the total concentration of all detectable tricyclic antidepressants produces a response greater than the cutoff of 300 ng/mL nortriptyline. This is a screening test, results are unconfirmed. Unconfirmed results are to be used for medical (treatment) purposes only. Tricyclic antidepressants detectable with this assay include: amitriptyline, clomipramine, desipramine, doxepin, imipramine, nortriptyline, protriptyline, and trimipramine.

Levels of tricyclic antidepressants in the therapeutic range may not be detectable.  

False-positive results may occur with the following drugs: Seroquel (quetiapine fumarate), Trileptal (oxcarbazepine), Benadryl (diphenhydramine) at toxic concentrations, Flexeril (cyclobenzaprine), Thioridazine, and Thorazine (chlorpromazine).

Compliance Category

FDA

Note
Hotline History
N/A
CPT Codes

80307

Components
Component Test Code* Component Chart Name LOINC
0090307 Tricyclic Antidepressant Detection 4073-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • amitriptyline
  • desipramine
  • domipramine
  • doxepin
  • imipramine
  • nortriptyline
  • protriptyline
  • Tricyclic Antidepressants, Total, detection
  • trimipramine
Tricyclic Antidepressant Detection