Ordering Recommendation

Optimize drug therapy and monitor patient adherence.

Mnemonic
AMIT/NORT
Methodology

Quantitative Liquid Chromatography-Tandem Mass Spectrometry

Performed

Mon, Wed, Fri

Reported

1-5 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Timing of specimen collection: Pre-dose (trough) draw - At steady state concentration.

Collect

Plain red. Also acceptable: Lavender (K2 or K3EDTA) or pink (K2EDTA).

Specimen Preparation

Separate serum or plasma from cells within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Whole blood. Gel separator tubes, light blue (citrate), or yellow (SPS or ACD solution).

Remarks
Stability

After separation from cells: Ambient: 5 days; Refrigerated: 2 weeks; Frozen: 6 months

Reference Interval

Effective February 19, 2013

Therapeutic Range Total (amitriptyline and nortriptyline): 95-250 ng/mL
Toxic Level Greater than 500 ng/mL

Interpretive Data

Toxic concentrations may cause anticholinergic effects, cardiac abnormalities and seizures.

Compliance Category

Laboratory Developed Test (LDT)

Note

Report includes individual values for amitriptyline, nortriptyline, and total.

Hotline History
N/A
CPT Codes

80335 (Alt code: G0480)

Components
Component Test Code* Component Chart Name LOINC
0090020 Amitriptyline/Nortriptyline, Total, SP 3335-7
0090024 Amitriptyline, SP 3333-2
0090210 Nortriptyline, SP 3872-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Amitriptyline/Nortriptyline Fractionation
  • Elatrol
  • Elavil
  • Endep
  • Etrafon
  • Laroxyl
  • Limbitrol
  • Saroten
  • Trepiline
  • Triavil
  • Tryptizol
  • Vanatrip
Amitriptyline and Nortriptyline, Serum or Plasma