Amitriptyline and Nortriptyline, Serum or Plasma
Ordering Recommendation
Use to optimize dosing and monitor patient adherence.
New York DOH Approval Status
Specimen Required
Timing of specimen collection: Predose (trough) draw at steady-state concentration.
Plain red. Also acceptable: Lavender (K2 or K3EDTA) or pink (K2EDTA).
Separate serum or plasma from cells within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP standard transport tube. (Min: 0.5 mL)
Refrigerated.
Whole blood. Gel separator tubes, light blue (citrate), or yellow (SPS or ACD solution).
After separation from cells: Ambient: 5 days; Refrigerated: 2 weeks; Frozen: 6 months
Methodology
Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Performed
Mon, Wed, Fri
Reported
1-7 days
Reference Interval
Effective February 19, 2013
Therapeutic Range | Total (amitriptyline and nortriptyline): 95-250 ng/mL |
Toxic Level | Greater than 500 ng/mL |
Interpretive Data
Toxic concentrations may cause anticholinergic effects, cardiac abnormalities and seizures.
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Laboratory Developed Test (LDT)
Note
Report includes individual values for amitriptyline, nortriptyline, and total.
Hotline History
Hotline History
CPT Codes
80335 (Alt code: G0480)
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
0090020 | Amitriptyline/Nortriptyline, Total, SP | 3335-7 |
0090024 | Amitriptyline, SP | 3333-2 |
0090210 | Nortriptyline, SP | 3872-9 |
Aliases
- Amitriptyline/Nortriptyline Fractionation
- Elatrol
- Elavil
- Endep
- Etrafon
- Laroxyl
- Limbitrol
- Saroten
- Trepiline
- Triavil
- Tryptizol
- Vanatrip