Ordering Recommendation

Aid in assessment of the etiology of anion gap acidosis. Determine whether ethylene glycol poisoning exists.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Timing of specimen collection: Dependent on time of exposure - test upon presentation to hospital.


Plain red. Also acceptable: Lavender (K2 or K3EDTA) or pink (K2EDTA).

Specimen Preparation

Separate serum or plasma from cells within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL) When drawing a blood specimen for ethylene glycol testing, use a nonalcohol-based cleanser at the venipuncture site.

Storage/Transport Temperature


Unacceptable Conditions

Whole blood. Gel separator tubes, light blue (citrate), or yellow (SPS or ACD solution).


After separation from cells: Ambient: 1 week; Refrigerated: 1 week; Frozen: 1 month


Quantitative Enzymatic Assay




1-4 days

Reference Interval

Effective February 19, 2013

Therapeutic Range No therapeutic range - Limit of detection 5 mg/dL
Toxic Level Greater than 20 mg/dL

Interpretive Data

Toxic concentrations may cause intoxication, CNS depression, metabolic acidosis, renal damage and hypocalcemia. Ethylene glycol is extremely toxic. Ingestion can be fatal if patients do not receive immediate medical treatment.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0090110 Ethylene Glycol 5646-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Antifreeze
  • Ethane-1, 2-Diol
Ethylene Glycol