Ordering Recommendation

May be used as a marker of severe combined immunodeficiency (SCID); lack of adenosine deaminase (ADA) allows deoxyadenosine to accumulate and kill lymphocytes.

Mnemonic
ADA
Methodology

Kinetic Spectrophotometry

Performed

Sun, Tue, Thu

Reported

1-4 days

New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Specimen Required
Patient Preparation
Collect

Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation

Do not freeze. Transport 1 mL whole blood.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Hemolyzed specimens.

Remarks
Stability

Ambient: 15 days; Refrigerated: 15 days; Frozen: Unacceptable

Reference Interval

Effective August 20, 2012

400-900 mU/g Hb

Interpretive Data

Adenosine Deaminase (ADA) deficiency is an autosomal recessive disorder of purine metabolism primarily affecting lymphocyte development, viability, and function. Affected individuals have less than 1 percent of normal ADA catalytic activity in red cell hemolysates. ADA deficiency is the cause of 20-30 percent of SCID cases. If the patient has been recently transfused, ADA deficiency may be masked; interpret results with caution. Heterozygotes cannot be identified by this test.

Compliance Category

Laboratory Developed Test (LDT)

Note
Hotline History
N/A
CPT Codes

84311

Components
Component Test Code* Component Chart Name LOINC
0083001 Adenosine Deaminase RBC 47549-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • ADA
Adenosine Deaminase, RBC