Ordering Recommendation

Surveillance and monitoring of hepatocellular carcinoma.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum separator tube.

Specimen Preparation

Allow specimen to clot completely at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature


Unacceptable Conditions



After separation from cells: Ambient: 8 hours; Refrigerated: 5 days; Frozen: 3 months (avoid repeated freeze/thaw cycles)


Quantitative Liquid Chromatography/Immunoassay


Mon, Thu


1-5 days

Reference Interval

By report

Test Number
Reference Interval
  Alpha Fetoprotein Total 0-15 ng/mL
  Alpha Fetoprotein L3 Pct 0.0-9.9 percent

Interpretive Data

The µTASWako method is used. Results obtained with different assay methods or kits cannot be used interchangeably. The AFP L3 Percent assay is intended as a risk assessment for the development of hepatocellular carcinoma in patients with chronic liver diseases. Patients with elevated serum AFP-L3 percent should be more intensely evaluated for evidence of hepatocellular carcinoma since elevated values have been shown to be associated with a seven-fold increase in the risk for developing hepatocellular carcinoma within 21 months. Results cannot be interpreted as absolute evidence of the presence or absence of malignant disease. For pregnant females, the result is not interpretable as a tumor marker.

Compliance Category



Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0081207 Alpha Fetoprotein Total 1834-1
0081209 Alpha Fetoprotein L3 Pct 42332-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • AFP
  • AFP-L3%
  • Alpha Fetoprotein
  • Alpha Fetoprotein, Total and L3 Percent
  • Alpha-Fetoprotein (AFP) L3% and Total, Hepatocellular Carcinoma Tumor Marker, Serum
  • Alpha-fetoprotein, Total
  • Hepatocellular Carcinoma AFP
  • Total AFP
Alpha Fetoprotein, Total and L3 Percent