Ordering Recommendation
Most sensitive test for detection of hyperandrogenemia in women and children. Acceptable test for androgen deficiency in men.
Mnemonic
Methodology
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry/Electrochemiluminescent Immunoassay
Total Testosterone and SHBG are measured and free testosterone is estimated from these measurements.
Performed
Sun-Sat
Reported
1-4 days
New York DOH Approval Status
Specimen Required
Collect between 6-10 a.m.
Serum separator tube or green (sodium or lithium heparin).
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.8 mL)
Refrigerated.
EDTA plasma.
This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS.
After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months
Reference Interval
Female Free Testosterone, pg/mL |
Male Free Testosterone, pg/mL |
---|---|
1-6 years: Less than 0.6 pg/mL 7-9 years: 0.6-1.8 pg/mL 10-11 years: 0.1-3.5 pg/mL 12-13 years: 0.9-6.8 pg/mL 14-15 years: 1.2-7.5 pg/mL 16-17 years: 1.2-9.9 pg/mL 18-30 years: 0.8-7.4 pg/mL 31-40 years: 1.3-9.2 pg/mL 41-51 years: 1.1-5.8 pg/mL Postmenopausal: 0.6-3.8 pg/mL Tanner Stage I: Less than 2.2 pg/mL Tanner Stage II: 0.4-4.5 pg/mL Tanner Stage III: 1.3-7.5 pg mL Tanner Stage IV: 1.1-15.5 pg/mL Tanner Stage V: 0.8-9.2 pg/mL |
1-6 years: Less than 0.6 pg/mL 7-9 years: 0.1-0.9 pg/mL 10-11 years: 0.1-6.3 pg/mL 12-13 years: 0.5-98.0 pg/mL 14-15 years: 3-138.0 pg/mL 16-17 years: 38.0-173.0 pg/mL 18 years and older: 47-244 pg/mL Tanner Stage I: Less than or equal to 3.7 pg/mL Tanner Stage II: 0.3-21 pg/mL Tanner Stage III: 1.0-98.0 pg mL Tanner Stage IV: 35.0-169.0 pg/mL Tanner Stage V: 41.0-239.0 pg/mL |
Interpretive Data
The concentration of free testosterone is derived from a mathematical expression based on the constant for the binding of testosterone to SHBG.
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Laboratory Developed Test (LDT)
Note
Testosterone Free, Females and Children by tandem mass spectrometry, only provides a result for the free testosterone value (pg/mL).
Hotline History
Hotline History
CPT Codes
84402
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
0081096 | Testosterone, Free LC-MS/MS | 2991-8 |