Testosterone, Free (Adult Females, Children, or Individuals on Testosterone-Suppressing Hormone Therapy)
Ordering Recommendation
Provides a calculated value for free testosterone concentration using total testosterone measured by mass spectrometry. May be used to evaluate hyperandrogenism in children and cisgender females, monitor testosterone-suppressing hormone therapies (eg, antiandrogens or estrogens), evaluate testosterone status in individuals with protein-binding abnormalities, or evaluate hypogonadism in cisgender males.
Mnemonic
Methodology
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry/Electrochemiluminescent Immunoassay
Total Testosterone and SHBG are measured and free testosterone is estimated from these measurements.
Performed
Sun-Sat
Reported
1-4 days
New York DOH Approval Status
Specimen Required
Collect between 6-10 a.m.
Serum separator tube or green (sodium or lithium heparin).
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.8 mL)
Refrigerated.
EDTA plasma.
After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months
Reference Interval
Female Free Testosterone, pg/mL |
Male Free Testosterone, pg/mL |
---|---|
1-6 years: Less than 0.6 pg/mL 7-9 years: 0.6-1.8 pg/mL 10-11 years: 0.1-3.5 pg/mL 12-13 years: 0.9-6.8 pg/mL 14-15 years: 1.2-7.5 pg/mL 16-17 years: 1.2-9.9 pg/mL 18-30 years: 0.8-7.4 pg/mL 31-40 years: 1.3-9.2 pg/mL 41-51 years: 1.1-5.8 pg/mL Postmenopausal: 0.6-3.8 pg/mL Tanner Stage I: Less than 2.2 pg/mL Tanner Stage II: 0.4-4.5 pg/mL Tanner Stage III: 1.3-7.5 pg mL Tanner Stage IV: 1.1-15.5 pg/mL Tanner Stage V: 0.8-9.2 pg/mL |
1-6 years: Less than 0.6 pg/mL 7-9 years: 0.1-0.9 pg/mL 10-11 years: 0.1-6.3 pg/mL 12-13 years: 0.5-98.0 pg/mL 14-15 years: 3-138.0 pg/mL 16-17 years: 38.0-173.0 pg/mL 18 years and older: 47-244 pg/mL Tanner Stage I: Less than or equal to 3.7 pg/mL Tanner Stage II: 0.3-21 pg/mL Tanner Stage III: 1.0-98.0 pg mL Tanner Stage IV: 35.0-169.0 pg/mL Tanner Stage V: 41.0-239.0 pg/mL |
Interpretive Data
Free testosterone concentration is calculated using total testosterone (measured by mass spectrometry) and the binding constant of testosterone and sex hormone-binding globulin (SHBG).
For individuals on testosterone-suppressing hormone therapies (e.g., antiandrogens or estrogens), refer to cisgender female reference intervals. For a complete set of all established reference intervals, refer to ltd.aruplab.com/Tests/Pub/0081059.
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Laboratory Developed Test (LDT)
Note
Hotline History
CPT Codes
84402
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
0081096 | Testosterone, Free by Mass Spec | 49042-5 |