Ordering Recommendation

May be useful for evaluating calcium metabolism in individuals with hypercalcemia or renal failure in addition to Vitamin D, 25-Hydroxy testing. Test is not appropriate for diagnosing vitamin D deficiency or insufficiency.

VIT D 1,25

Quantitative Chemiluminescent Immunoassay




Within 24 hours

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Serum separator tube or plain red, lithium heparin or EDTA plasma.

Specimen Preparation

Allow serum separator or plain red tube to sit for 15-20 minutes at room temperature for proper clot formation. Centrifuge and separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)
Storage/Transport Temperature: Refrigerated.

Storage/Transport Temperature


Unacceptable Conditions

Grossly hemolyzed or lipemic specimens.


After separation from cells: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 6 months

Reference Interval

19.9-79.3 pg/mL

Interpretive Data

This test is primarily indicated during patient evaluation for hypercalcemia and renal failure. A normal result does not rule out Vitamin D deficiency. The recommended test for diagnosing Vitamin D deficiency is Vitamin D 25-hydroxy. 

No compliance statements are in use for this test.

Hotline History
CPT Codes


Component Test Code* Component Chart Name LOINC
0080385 Vitamin D, 1,25-Dihydroxy 1649-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • 1,25-(OH)2-D
  • 1,25-Dihydroxy Vitamin D
  • 1,25-Dihydroxycholecalciferol
  • 1,25-Dihydroxyvitamin D
  • 1,25-Dihydroxyvitamin D3
  • Calcitriol
  • D, 1-25 Dihydroxy, Vitamin
  • Vit D 1,25
Vitamin D, 1, 25-Dihydroxy