An isolated pyruvic acid concentration has little clinical value. Preferred test is Lactate to Pyruvate Ratio, Whole Blood (2007935), which reports concentrations for lactate, pyruvate, and L:P ratio on the same specimen.
Quantitative Enzymatic Assay
New York DOH Approval Status
Patient should be fasting and at complete rest. Patient should avoid any exercise of the arm or hand before or during collection. Draw the specimen without the use of a tourniquet or within three minutes of applying the tourniquet, but before releasing the tourniquet.
Green (Sodium or Lithium Heparin).
If whole blood is collected in a syringe, transfer immediately to green (sodium or lithium heparin) tube before preparing specimen.
1) Immediately after blood is drawn, add exactly 1 mL whole blood to a chilled pyruvate collection tube containing 2 mL 8 percent (w/v) perchloric acid (ARUP supply #16567) available online through eSupply using ARUP Connect™ or contact Client Services at (800) 522-2787.
2) Mix well for 30 seconds then place in an ice bath for 10 minutes.
3) Centrifuge for 10 minutes at 1500 x g.
4) Decant 2 mL supernatant to an ARUP Standard Transport Tube and freeze. (Min: 1 mL)
Ambient: Unacceptable; Refrigerated: 2 days; Frozen: 4 weeks
0.030-0.107 mmol/L (venous blood)
If less than 1 mL of blood is added to collection tube, pH of the supernatant will be too low for testing.
|Component Test Code*||Component Chart Name||LOINC|
- Pyruvic Acid