Ordering Recommendation

An isolated pyruvic acid concentration has little clinical value.  Preferred test is Lactate to Pyruvate Ratio, Whole Blood (2007935), which reports concentrations for lactate, pyruvate, and L:P ratio on the same specimen.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Patient should be fasting and at complete rest. Patient should avoid any exercise of the arm or hand before or during collection. Draw the specimen without the use of a tourniquet or within three minutes of applying the tourniquet, but before releasing the tourniquet.


Green (Sodium or Lithium Heparin).

Specimen Preparation

If whole blood is collected in a syringe, transfer immediately to green (sodium or lithium heparin) tube before preparing specimen.
1) Immediately after blood is drawn, add exactly 1 mL whole blood to a chilled pyruvate collection tube containing 2 mL 8 percent (w/v) perchloric acid (ARUP supply #16567) available online through eSupply using ARUP Connect™ or contact Client Services at (800) 522-2787.
2) Mix well for 30 seconds then place in an ice bath for 10 minutes.
3) Centrifuge for 10 minutes at 1500 x g.
4) Decant 2 mL supernatant to an ARUP Standard Transport Tube and freeze. (Min: 1 mL)

Storage/Transport Temperature


Unacceptable Conditions

Ambient: Unacceptable; Refrigerated: 2 days; Frozen: 4 weeks


Quantitative Enzymatic Assay




1-2 days

Reference Interval

0.030-0.107 mmol/L (venous blood)

Interpretive Data

Compliance Category



If less than 1 mL of blood is added to collection tube, pH of the supernatant will be too low for testing.

Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0080310 Pyruvic Acid 14121-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Pyruvate
  • Pyruvic Acid
Pyruvic Acid