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Glucose-6-Phosphate Dehydrogenase
Ordering Recommendation
Preferred initial screening test for G6PD deficiency. For genetic testing in individuals of African descent, refer to Glucose-6-Phosphate Dehydrogenase (G6PD) 2 Mutations (0051684); for genetic testing in individuals with other high-risk ethnic backgrounds, refer to Glucose-6-Phosphate Dehydrogenase Deficiency (G6PD) Sequencing (3004457).
New York DOH Approval Status
Specimen Required
Yellow (ACD solution A). Also acceptable: Green (sodium or lithium heparin), lavender (K2EDTA or K3EDTA), or pink (K2EDTA). Enzyme most stable in acid citrate dextrose (ACD).
Do not freeze. Transport 3 mL whole blood. (Min: 1.5 mL heparin or EDTA collection tube; Min: 0.5 mL pediatric collection tube).
Refrigerated
Clotted, frozen, or hemolyzed specimens.
Pediatric minimum 0.5 mL if collected and transported in a pediatric collection K2EDTA tube. ACD collection tubes should be filled to maximum collectible volume and are not recommended for pediatric specimen collection or preservation.
ARUP G6PD results are normalized to Hemoglobin. Reporting units are U/g Hb. Alternative methods may normalize G6PD results to red blood cells and use different reporting units.
Room Temperature: 8 hours; Refrigerated: 1 week; Frozen: Unacceptable
Methodology
Quantitative Enzymatic Assay
Performed
Sun-Sat
Reported
1-3 days
Reference Interval
| Test Number |
Components |
Reference Interval |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Glucose-6-Phosphate Dehydrogenase |
|
Interpretive Data
World Health Organization guidance on classification of G6PD activity is as follows:
Males: G6PD activity less than 30% of the normal median are regarded as G6PD deficient. Males with G6PD activity of 30% or more of the normal median can be regarded as G6PD normal.
Females: G6PD activity less than 30% of the normal median are regarded as G6PD deficient. Females with G6PD activity of 80% or more of the normal median can be regarded as G6PD normal. G6PD activity between 30% and 80% of the normal median are regarded as intermediate activity.
Cutoffs and results are specific to this G6PD assay and configuration and cannot be used interchangeably across different assays, parameters, and/or instrument configurations.
Reference: Guide to G6PD deficiency rapid diagnostic testing to support P. vivax radical cure. Geneva: World Health Organization; 2018. ISBN 978-92-4-151428-6
| Percent of Normal Activity (U/g Hb) | |||
|---|---|---|---|
| Age |
100% |
80% |
30% |
| <8 days | 19.7 | 15.8 | 5.9 |
| 8 - 30 days | 18.2 | 14.6 | 5.5 |
| 1 - 6 months | 16.1 | 12.9 | 4.8 |
| 7 - 12 months | 13.8 | 11.0 | 4.1 |
| 1 - 17 years | 12.9 | 10.3 | 3.9 |
| 18 years | 12.7 | 10.2 | 3.8 |
| Percentage of normal activity cutoffs to G6PD enzyme activity. 100% for males and females is defined as the 50th percentile of non-affected males. | |||
FDA
Note
Patients who have recently received transfusions have normal donor cells that may mask G-6-PD deficient erythrocytes.
Hotline History
Hotline History
CPT Codes
82955
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 0080135 | Glucose-6-Phosphate Dehydrogenase | 32546-4 |
Aliases
- G-6-PD
- G6PD Erythrocytes
- RBC G6PD test
