Ordering Recommendation

Use for nutritional assessment of vitamin B6.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Collect specimen after an overnight fast.


Green (Sodium or Lithium Heparin), Lavender (EDTA), Pink (K2 EDTA), Plasma Separator Tube (PST), Serum Separator Tube (SST), or Plain Red.

Specimen Preparation

Separate plasma or serum from cells, protect from light and transfer 1 mL plasma or serum to an ARUP Amber Transport Tube within 1 hour of collection. (Min: 0.5 mL) Separate light-protected specimens must be submitted when multiple tests are ordered.

Storage/Transport Temperature


Unacceptable Conditions

Whole blood. Specimens not protected from light. Icteric specimens.


After separation from cells: Ambient: 3 Hours; Refrigerated: 1 week; Frozen: 2 months


Quantitative Liquid Chromatography-Tandem Mass Spectrometry




1-5 days

Reference Interval

20-125 nmol/L

Interpretive Data

Pyridoxal 5'-phosphate measured in a specimen collected following an 8 hour or overnight fast accurately indicates vitamin B6 nutritional status. Non-fasting specimen concentration reflects recent vitamin intake.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


This test measures pyridoxal 5-phosphate, the biologically active form of vitamin B6.

Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0080111 Vitamin B6 (Pyridoxal 5-Phosphate) 62236-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • P 5-P
  • Pyridoxal Phosphate
Vitamin B[6] (Pyridoxal 5-Phosphate)