Testosterone, Bioavailable and Total, Includes Sex Hormone-Binding Globulin (Adult Males or Individuals on Testosterone Hormone Therapy)
Provides a calculated value for bioavailable testosterone concentration using total testosterone measured by immunoassay. Use to evaluate hypogonadism in cisgender males with a total testosterone concentration at the lower limit of normal. May be used to evaluate testosterone status in individuals with protein-binding abnormalities or to monitor testosterone hormone therapies. Not recommended when low testosterone concentrations, such as those found in children and cisgender females, are expected. For these individuals, testing by mass spectrometry is recommended; refer to Testosterone, Bioavailable and Total, Includes Sex Hormone-Binding Globulin (Adult Females, Children, or Individuals on Testosterone-Suppressing Hormone Therapy) (0081057).
To compare this test to other testosterone tests, refer to the ARUP Testosterone Tests Comparison table.
New York DOH Approval Status
Collect specimen between 6-10 a.m.
Serum separator tube or green (lithium heparin).
Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.6 mL)
After separation from cells: Ambient: 48 hours; Refrigerated: 1 week; Frozen: 2 months
Quantitative Electrochemiluminescent Immunoassay/Calculation
Within 24 hours
|Testosterone, Free Calculation||
|Testosterone, Percentage Free||
|Testosterone by Immunoassay||
|Sex Hormone Binding Globulin||
Bioavailable testosterone concentration is calculated using total testosterone (measured by immunoassay) and the binding constant of testosterone and sex hormone-binding globulin (SHBG) and/or albumin. Testosterone immunoassays are both imprecise and inaccurate at low testosterone concentrations, such as those found in children and cisgender females. For these individuals, testing by mass spectrometry is recommended; refer to Testosterone, Bioavailable and Total, Includes Sex Hormone-Binding Globulin (Adult Females, Children, or Individuals on Testosterone-Suppressing Hormone Therapy) (ARUP test code 0081057).
For individuals on testosterone hormone therapy, refer to cisgender male reference intervals. No reference intervals have been established for males younger than 14 years or for cisgender females. For a complete set of all established reference intervals, refer to ltd.aruplab.com/Tests/Pub/0070102.
Bioavailable testosterone includes free plus weakly bound (non-SHBG bound) testosterone. Bioavailable testosterone is an assessment of the biologically active testosterone in serum.
The concentrations of free and bioavailable testosterone are derived from mathematical expressions based on constants for the binding of testosterone to albumin and/or sex hormone binding globulin.
84402; 84403; 84270
|Component Test Code*||Component Chart Name||LOINC|
|0070113||Testosterone, Free Calculation||2991-8|
|0070114||Testosterone, Percentage Free||15432-8|
|0070130||Testosterone by Immunoassay||2986-8|
|0099375||Sex Hormone Binding Globulin||13967-5|
- Testosterone-estrogen Binding Globulin