Ordering Recommendation

Acceptable test for the evaluation of suspected hypogonadism in men. Not recommended for use in women and children.




Quantitative Electrochemiluminescent Immunoassay
The concentrations of free and bioavailable testosterone are derived from mathematical expressions based on constants for the binding of testosterone to albumin and/or sex hormone binding globulin.




Within 24 hours

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Collect specimen between 6-10 a.m.


Serum separator tube or green (lithium heparin).

Specimen Preparation

Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.6 mL)

Storage/Transport Temperature


Unacceptable Conditions

EDTA plasma.


After separation from cells: Ambient: 48 hours; Refrigerated: 1 week; Frozen: 2 months

Reference Interval

Test Number
Reference Interval
0070111 Testosterone Free, Adult Male Male:
14-15 years:  3-138 pg/mL
16-17 years:  38-173 pg/mL
18 years and older: 47-244 pg/mL

Tanner Stage IV:  35-169 pg/mL
Tanner Stage V:  41-239 pg/mL
0070130 Testosterone, Adult Male Male:
14-15 years: 33-585 ng/dL
16-17 years: 185-886 ng/dL
18-39 years: 300-1080 ng/dL
40-59 years: 300-890 ng/dL
60 years and older: 300-720 ng/dL

Tanner Stage IV: 165-854 ng/dL
Tanner Stage V: 194-783 ng/dL
0099375 Sex Hormone Binding Globulin
Age Male Female
1-30 days
31-364 days
1-3 years
4-6 years
7-9 years
10-12 years
13-15 years
16-17 years
18 years and older
13-85 nmol/L
70-250 nmol/L
50-180 nmol/L
45-175 nmol/L
28-190 nmol/L
23-160 nmol/L
13-140 nmol/L
10-60 nmol/L
11-80 nmol/L
14-60 nmol/L
60-215 nmol/L
60-190 nmol/L
55-170 nmol/L
35-170 nmol/L
17-155 nmol/L
11-120 nmol/L
19-145 nmol/L
30-135 nmol/L
Tanner Stage I
Tanner Stage II
Tanner Stage III
Tanner Stage IV
Tanner Stage V
26-186 nmol/L
22-169 nmol/L
13-104 nmol/L
11-60 nmol/L
11-71 nmol/L
30-173 nmol/L
16-127 nmol/L
12-98 nmol/L
14-151 nmol/L
23-165 nmol/L

Reference Interval
Testosterone, Bioavailable, Adult Male 14-15 years:  10-337 ng/dL
16-17 years: 35-509 ng/dL
18 years and older: 131-682 ng/dL

Tanner Stage IV:  40-485 ng/dL
Tanner Stage V:  124-596 ng/dL
Testosterone, Percentage Free, Adult Male 1.6-2.9%

Interpretive Data

Bioavailable testosterone concentration is calculated using total testosterone (measured by immunoassay) and the binding constant of testosterone and sex hormone binding globulin (SHBG) and/or albumin.  Testosterone immunoassys are both imprecise and inaccurate at low testosterone concentrations, usch as those found in children and cisgender females.  For these individuals testing by mass spectrometry is reccomended; refer to Testosteron, Bioavaialble and Total, includes Sex-Hormone Binding Globulin (Adult Females, Children or Individual on Testosterone Supressing Hormone Therapy) (ARUP test code 0081057).

For individuals of testosterone hormone therapy refer to cisgender male reference intervals.  No reference intervals have been establised for males youger then 14 years or for cisgender females.  For a complete set of all established reference interals, refer to ltd.aruplab.com/Tests/Pub/0070102.

The concentration of Free Testosterone is derived from a mathematical expression based on the constant for the binding of testosterone to Sex Hormone Binding Globulin (SHBG).

Compliance Category



Plese refer to individual components for stability fot this test.  This test is not reccommended for females or children.  For testsing on females or children refer to Testoterone, Bioavailable and Toal, includes Sex Hormone Binding Globuln (adult Females, Children, or Individulas on Tetosterone Supressing Hormone Therapy) Testosterone, Bioavaliable & Sex Hormone Binding Globulin (includes Total Testosterone), Females or Children (0081057).

Hotline History


CPT Codes

84402; 84403; 84270


Component Test Code* Component Chart Name LOINC
0070113 Testosterone, Free Calculation 2991-8
0070114 Testosterone, Percentage Free 15432-8
0070130 Testosterone, Adult Male 2986-8
0070134 Testosterone, Bioavailable 2990-0
0099375 Sex Hormone Binding Globulin 13967-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • TeBG
  • Testosterone-estrogen Binding Globulin
Testosterone, Bioavailable and Sex Hormone Binding Globulin (Includes Total Testosterone), Adult Male