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Acid-Fast Bacillus (AFB) Identification
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Identify mycobacterial isolates, including requests for speciation within the M. tuberculosis complex.
Identification. Methods may include biochemical, mass spectrometry, nucleic acid probe, polymerase chain reaction, or sequencing.
Sun-Sat
Varies (depends on organism growth)
Actively growing organism, in pure culture.
Place each specimen in an individually sealed bag. Transport sealed container with pure culture on solid or in liquid media.
Room temperature. If culture is suspected of being a microorganism identified on the IATA list as an infectious substance affecting humans, submit specimen according to Infectious Substance, Category A, shipping guidelines.
Mixed cultures or non-viable organisms. Organisms submitted on an agar plate.
Indicate source of organism and any other pertinent information, such as the growth rate of the organism or DNA probe results.
Ambient: 2 weeks; Refrigerated: 2 weeks; Frozen: 2 weeks
Complete identification of clinically significant isolates.
For identification by DNA sequencing and MALDI-TOF:
Laboratory Developed Test (LDT)
If the isolate M. tuberculosis complex is to be speciated, enter this information in the comment field and ship Category A.
A processing fee will be billed for all organisms submitted that are not in pure culture, as indicated in specimen requirements.
Identification of AFB isolates is billed by individual tests performed.
Probes can be performed directly from Middlebrook 7H9 broth. MGIT is not suitable for direct probe testing.
CPT codes for identification vary based on method.
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 0060999 | AFB Identification | 543-9 |
- AFB identification by sequencing, 16S rRNA
- AFBSEQ
- M. tuberculosis identification
- MTBGP
- Mycobacterium tuberculosis Probe
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