Pyrazinamide (PZA) testing is suspended due to issues with the manufacturer’s kit. ARUP will continue testing the other first-line drugs: rifampin, isoniazid, and ethambutol. If PZA susceptibility testing is required during this test delay, contact your local public health laboratory or the CDC for pncA gene testing for PZA resistance mutations.

0060997
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Acid-Fast Bacillus (AFB) Identification with Reflex to Susceptibility

MC AFBIS
Example Reports
Standard

Ordering Recommendation

Identify M. tuberculosis complex species and susceptibility.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Actively growing organism, in pure culture.

Specimen Preparation

Transport sealed container with pure culture on solid or in liquid media. Place each specimen in an individually sealed bag.

Storage/Transport Temperature

Room temperature. If culture is suspected of being a microorganism identified on the IATA list as an infectious substance affecting humans, submit specimen according to Infectious Substance, Category A, shipping guidelines.

Unacceptable Conditions

Mixed cultures or non-viable organisms. Organisms submitted on agar plate.

Remarks

Indicate source of organism and any other pertinent information such as growth rate of the organism or DNA probe results.

Stability

Ambient: 2 weeks; Refrigerated: 2 weeks; Frozen: 2 weeks

Methodology

Methods may include biochemical, mass spectrometry, or sequencing.
Susceptibility Testing

Performed

Sun-Sat

Reported

Varies
Depends on organism growth.

Reference Interval

Complete identification and susceptibility of clinically significant isolates of M. tuberculosis complex, M. kansasii, M. avium-intracellulare complex, M. fortuitum complex, M. abscessus complex, M. chelonae, M. immunogenum and any isolate from a significant source.

Interpretive Data

For identification by DNA sequencing and MALDI-TOF

Compliance Category

Depends on Specimen/Source/Method

Note

If a significant organism listed above is identified, then the appropriate susceptibility panel will be added. Additional charges apply. For other organisms, contact ARUP if susceptibility testing is clinically indicated.

A processing fee will be billed for all organisms submitted that are not in pure culture as requested in the specimen requirements. Identification of AFB isolates is billed by individual tests performed.

Hotline History

N/A

CPT Codes

CPT codes for identification and susceptibility vary based on method.

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Components

Component Test Code* Component Chart Name LOINC
0060997 AFB Identification/Susceptibility 29579-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Primary Panel
  • MA AFB
  • Mycobacterium tuberculosis identification
  • Mycobacteria identification
  • ID by sequencing
  • M. tuberculosis identification
  • Primary Anti-tuberculosis agents
Acid-Fast Bacillus (AFB) Identification with Reflex to Susceptibility