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Human Metapneumovirus DFA
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Ordering Recommendation
Preferred initial test to detect human metapneumovirus (hMPV) in children and adults.
Mnemonic
Methodology
Direct Fluorescent Antibody Stain
Performed
Sun-Sat
Reported
Within 24 hours
New York DOH Approval Status
Specimen Required
Respiratory specimen: Bronchoalveolar lavage (BAL), nasopharyngeal (aspirate, swab, or washing), or tracheal aspirate.
Do not freeze.
Fluid: Transfer 3 mL specimen to a sterile container. (Min: 0.5 mL) Also acceptable: Transfer to 3 mL viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Swab: Place in 3 mL viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Refrigerated.
Calcium alginate, eSwab, dry, or wood swabs. Slides.
Specimen source preferred.
Ambient: 2 hours; Refrigerated: 72 hours; Frozen: Unacceptable
Reference Interval
Negative
Interpretive Data
FDA
Note
Sensitivity of DFA methodology is dependent upon adequacy of the specimen. If there are fewer than 20 cells, the DFA result will be reported as "sample inadequate."
Hotline History
CPT Codes
87299
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 0060778 | Human Metapneumovirus DFA | 40979-7 |
| 0060789 | Human Metapneumovirus DFA Source | 66746-9 |
Aliases
- Antigen detection
- Direct detection
- HMPV
- Metapneumovirus
- MPV
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