Detect varicella-zoster virus (VZV). DFA sensitivity is highest when performed on scrapings from base of lesions. Molecular testing is generally preferred; refer to Varicella-Zoster Virus by PCR (0060042).
Direct Fluorescent Antibody Stain
Within 24 hours
Collect vesicle specimen during first 3 days of rash.
Vesicle swab, tissue, or skin scrapings. Swab should be rolled in base of fresh vesicle to obtain cells.
Immediately transfer specimen to viral transport media (ARUP Supply #12884) and refrigerate (extremely temperature sensitive). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Bone marrow, CSF, or whole blood. Calcium alginate, eSwab, dry or wood swabs.
Specimen source preferred.
Ambient: 4 hours; Refrigerated: 72 hours; Frozen: Unacceptable
Approximately 20% of samples submitted for VZV are positive for herpes simplex virus. Testing for herpes simplex virus is NOT included in this test.
Sensitivity of DFA methodology is dependent upon adequacy of the specimen. If there are fewer than 20 cells, the DFA result will be reported as "sample inadequate."
Culture is recommended for any DFA-negative swabs or inadequate samples. Refer to Varicella-Zoster Virus DFA with Reflex to Varicella-Zoster Virus Culture (0060282).
|Component Test Code*||Component Chart Name||LOINC|
|0060290||Varicella-Zoster Virus by DFA||5882-6|
|0060341||Varicella-Zoster Virus FA Source||31208-2|
- Herpes Zoster Virus
- Varicella Zoster Virus
- VZV Antigen Detection