Ordering Recommendation

Detect varicella-zoster virus (VZV). DFA sensitivity is highest when performed on scrapings from base of lesions. Molecular testing is generally preferred; refer to Varicella-Zoster Virus by PCR (0060042).

Mnemonic
VZVFA
Methodology

Direct Fluorescent Antibody Stain

Performed

Sun-Sat

Reported

Within 24 hours

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Collect vesicle specimen during first 3 days of rash.

Collect

Vesicle swab, tissue, or skin scrapings. Swab should be rolled in base of fresh vesicle to obtain cells.

Specimen Preparation

Immediately transfer specimen to viral transport media (ARUP Supply #12884) and refrigerate (extremely temperature sensitive).  Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Bone marrow, CSF, or whole blood. Calcium alginate, eSwab, dry or wood swabs.

Remarks

Specimen source preferred.

Stability

Ambient: 4 hours; Refrigerated: 72 hours; Frozen: Unacceptable

Reference Interval

Negative

Interpretive Data

Approximately 20% of samples submitted for VZV are positive for herpes simplex virus. Testing for herpes simplex virus is NOT included in this test.

Compliance Category

FDA

Note

Sensitivity of DFA methodology is dependent upon adequacy of the specimen.  If there are fewer than 20 cells, the DFA result will be reported as "sample inadequate."

Culture is recommended for any DFA-negative swabs or inadequate samples. Refer to Varicella-Zoster Virus DFA with Reflex to Varicella-Zoster Virus Culture (0060282).

Hotline History
N/A
CPT Codes

87290

Components
Component Test Code* Component Chart Name LOINC
0060290 Varicella-Zoster Virus by DFA 5882-6
0060341 Varicella-Zoster Virus FA Source 31208-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Herpes Zoster Virus
  • Varicella Zoster Virus
  • VZV
  • VZV Antigen Detection
Varicella-Zoster Virus DFA