Ordering Recommendation

Not a preferred stand-alone test.

Mnemonic
RSPFA
Methodology

Direct Fluorescent Antibody Stain

Performed

Sun-Sat

Reported

Within 24 hours

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Respiratory specimen: Bronchoalveolar lavage (BAL), nasopharyngeal (aspirate, swab, or washing), or tracheal aspirate.

Specimen Preparation

Fluid: Transfer 3 mL specimen to a sterile container. (Min: 0.5 mL) Also acceptable: Transfer to 3 mL viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Swab:
Place in 3 mL viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Dry swabs, eSwab. Slides.

Remarks

Specimen source preferred.

Stability

Ambient: 2 hours; Refrigerated: 72 hours; Frozen: Unacceptable

Reference Interval

Negative

Interpretive Data



No compliance statements are in use for this test.

Note

Viral culture of DFA-negative specimens is strongly recommended.  Refer to Respiratory Viruses DFA with Reflex to Viral Culture (0060281). The sensitivity of DFA testing for RSV is greater than 95% and does not require culture backup.

Hotline History
N/A
CPT Codes

87276; 87275; 87279 x3; 87280; 87260; 87299

Components
Component Test Code* Component Chart Name LOINC
0060308 Influenza A, DFA 5863-6
0060309 Influenza B, DFA 5867-7
0060311 Parainfluenza Type 1, DFA 5869-3
0060312 Parainfluenza Type 2, DFA 5871-9
0060313 Parainfluenza Type 3, DFA 5873-5
0060314 Adenovirus, DFA 5834-7
0060316 Respiratory Syncytial Virus, DFA 5877-6
0060340 RSPFA Source 31208-2
0060778 Human Metapneumovirus DFA 40979-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Adenovirus
  • HMPV
  • human mentapneumovirus by DFA
  • Influenza A
  • Influenza B, Parainfluenza Types 1, 2, 3,
  • Respiratory profile, direct detection DFA
  • Respiratory Virus Screen, DFA
  • RSV
Respiratory Viruses DFA