Ordering Recommendation

Not recommended for inpatients or immunocompromised individuals. Acceptable test for the detection of influenza in outpatients.

Mnemonic
FLUFAC
Methodology

Direct Fluorescent Antibody Stain/Cell Culture

Performed

Sun-Sat

Reported

Within 24 hours

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Respiratory specimen: Bronchoalveolar lavage (BAL), nasopharyngeal (aspirate, swab, or washing), or tracheal aspirate.

Specimen Preparation

Do not freeze. 
Fluid:
Transfer 3 mL specimen to a sterile container. (Min: 0.5 mL) Also acceptable: Transfer to 3 mL viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Swab:
Place in 3 mL viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Calcium alginate, eSwab, dry or wood swabs. Slides.

Remarks

Specimen source preferred.

Stability

Ambient: 1 hour; Refrigerated: 72 hours; Frozen: Unacceptable

Reference Interval

Negative

Interpretive Data



No compliance statements are in use for this test.

Note

If DFA is negative or inadequate, then an influenza culture (0060286) will be added. Additional charges apply.

Sensitivity of DFA methodology is dependent upon adequacy of the specimen.  If specimen contains fewer than 20 cells, then the DFA result will be reported as "inadequate." 

Hotline History
N/A
CPT Codes

87276; 87275; if reflexed, add 87254

Components
Component Test Code* Component Chart Name LOINC
0060306 Influenza A-DFA 5863-6
0060307 Influenza B-DFA 5867-7
0060337 Flufac Source 31208-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Flu Test
  • Influenza Antigen Test
  • Influenza by DFA
  • Influenza Virus Type A & B Antigen, DFA
Influenza Virus A and B DFA with Reflex to Influenza Virus A and B Rapid Culture