Detect varicella-zoster virus (VZV). Negative or inadequate DFA results are confirmed with culture. DFA sensitivity is highest when performed on scrapings from base of lesions; culture sensitivity is highest when specimens are collected soon after onset of symptoms. Molecular testing is generally preferred; refer to Varicella-Zoster Virus by PCR (0060042).
Direct Fluorescent Antibody Stain/Cell Culture
DFA: Within 24 hours; Culture: 3-5 days
Collect vesicle specimen during first three days of rash.
Vesicle fluid or swab, tissue, or skin scrapings. Swab should be rolled in base of fresh vesicle to obtain cells.
Immediately transfer specimen to viral transport media (ARUP Supply #12884) and refrigerate (extremely temperature sensitive). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Bone marrow CSF, or whole blood. Calcium alginate, eSwab, dry or wood swabs.
Specimen source preferred.
Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: Unacceptable
Approximately 20% of samples submitted for VZV are positive for herpes simplex virus. Testing for herpes simplex virus is NOT included in this test.
If DFA is negative or inadequate, then a VZV culture will be added. Additional charges apply.
Sensitivity of DFA methodology is dependent upon adequacy of the specimen. If specimen contains fewer than 20 cells, then the DFA result will be reported as "inadequate."
87290; if reflexed, add 87252; 87254
|Component Test Code*||Component Chart Name||LOINC|
|0060282||Varicella-Zoster Virus, DFA||5882-6|
|0060335||Varicella-Zoster Virus Fac Source|
- Herpes Zoster
- Herpes Zoster Antigen Detection & Culture
- Varicella Zoster Virus
- Varicella Zoster Virus Antigen Detection
- VZV DFA