Ordering Recommendation

Detect varicella-zoster virus (VZV). Negative or inadequate DFA results are confirmed with culture. DFA sensitivity is highest when performed on scrapings from base of lesions; culture sensitivity is highest when specimens are collected soon after onset of symptoms. Molecular testing is generally preferred; refer to Varicella-Zoster Virus by PCR (0060042).

Mnemonic
VZVFAC
Methodology

Direct Fluorescent Antibody Stain/Cell Culture

Performed

Sun-Sat

Reported

3-5 days
DFA: Within 24 hours; Culture: 3-5 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Collect vesicle specimen during first three days of rash.

Collect

Vesicle fluid or swab, tissue, or skin scrapings. Swab should be rolled in base of fresh vesicle to obtain cells.

Specimen Preparation

Immediately transfer specimen to viral transport media (ARUP Supply #12884) and refrigerate (extremely temperature sensitive). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Bone marrow CSF, or whole blood. Calcium alginate, eSwab, dry or wood swabs.

Remarks

Specimen source preferred.

Stability

Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: Unacceptable

Reference Interval

Negative

Interpretive Data

Approximately 20% of samples submitted for VZV are positive for herpes simplex virus.  Testing for herpes simplex virus is NOT included in this test.

Compliance Category

FDA

Note

If DFA is negative or inadequate, then a VZV culture will be added. Additional charges apply.

Sensitivity of DFA methodology is dependent upon adequacy of the specimen. If specimen contains fewer than 20 cells, then the DFA result will be reported as "inadequate."

Hotline History
N/A
CPT Codes

87290; if reflexed, add 87252; 87254

Components
Component Test Code* Component Chart Name LOINC
0060282 Varicella-Zoster Virus, DFA 5882-6
0060335 Varicella-Zoster Virus Fac Source
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Herpes Zoster
  • Herpes Zoster Antigen Detection & Culture
  • Varicella Zoster Virus
  • Varicella Zoster Virus Antigen Detection
  • VZV
  • VZV DFA
Varicella-Zoster Virus DFA with Reflex to Varicella-Zoster Virus Culture