Respiratory Viruses DFA with Reflex to Viral Culture, Respiratory

0060281
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Example Reports
Negative
Positive

Ordering Recommendation

Order this test when the detection of respiratory viruses in addition to influenza is important. Test detects influenza (A, B), parainfluenza (1, 2, 3), adenovirus, respiratory syncytial virus (RSV), and human metapneumovirus (hMPV). Molecular methods are preferred for immunocompromised and hospitalized patients. For general inpatient workup of influenza-like illness, order the Respiratory Virus Mini Panel by PCR (0060764).

Mnemonic

RSPFAC

Methodology

Direct Fluorescent Antibody Stain/Cell Culture

Performed

Sun-Sat

Reported

Within 24 hours

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Respiratory specimen: Bronchoalveolar lavage (BAL), nasopharyngeal (aspirate, swab, or washing), or tracheal aspirate.

Specimen Preparation

Fluid: Transfer 3 mL specimen to a sterile container. (Min: 0.5 mL) Also acceptable: Transfer to 3 mL viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Swab: Place in 3 mL viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Calcium alginate, dry or wood swabs. Slides.

Remarks

Specimen source preferred.

Stability

Ambient: 2 hours; Refrigerated: 72 hours; Frozen: Unacceptable

Reference Interval

Negative

Interpretive Data



Compliance Category

FDA

Note

If DFA is negative or inadequate, then a respiratory viral culture will be added. Additional charges apply.

Sensitivity of DFA methodology is dependent upon adequacy of the specimen. If specimen contains fewer than 20 cells, then the DFA result will be reported as "inadequate." 

Hotline History

N/A

CPT Codes

87276; 87275; 87279 x3; 87280; 87260; 87299; if reflexed add 87252; if definitive identification performed, add 87253.

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Components

Component Test Code* Component Chart Name LOINC
0060294 Influenza A - DFA 5863-6
0060295 Influenza B - DFA 53115-2
0060296 Parainfluenza Type 1 - DFA 5869-3
0060297 Parainfluenza Type 2 - DFA 5871-9
0060298 Parainfluenza Type 3 - DFA 5873-5
0060299 Adenovirus - DFA 5834-7
0060301 Respiratory Syncytial Virus - DFA 5877-6
0060334 RSP Fac Source 66746-9
0060778 Human Metapneumovirus DFA 40979-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Adenovirus
  • CMV culture
  • Culture for virus
  • cytomegalovirus
  • enterovirus culture
  • herpes simplex virus
  • HMPV
  • HSV culture
  • human mentapneumovirus by DFA
  • Influenza A
  • influenza A and B culture
  • Influenza B, Parainfluenza Types 1, 2, 3,
  • parainfluenza types 1, 2, 3
  • Respiratory profile, direct detection DFA
  • Respiratory Virus Screen, DFA
  • RSV
  • RSV culture
  • varicella-zoster virus
  • VSV culture
Respiratory Viruses DFA with Reflex to Viral Culture, Respiratory