Herpes Simplex Virus DFA with Reflex to Herpes Simplex Virus Culture

0060280
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Negative
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Ordering Recommendation

Rapid diagnostic test with culture backup for negative DFA results. May be helpful in identifying acute herpes simplex virus (HSV) infection in active lesions (eg, vesicles, ulcers, inflammation of mucous membranes). Molecular testing is generally preferred; refer to Herpes Simplex Virus (HSV-1/HSV-2) Subtype by PCR (2010095).

Mnemonic

HSVFAC

Methodology

Direct Fluorescent Antibody (DFA) Stain/Cell Culture

Performed

Sun-Sat

Reported

Within 24 hours

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Buccal mucosa lesion; cervical or genital lesion; eye, throat, or vesicle swab; bronchoalveolar lavage (BAL); tissue or vesicle fluid.

Specimen Preparation

Fluid: Transfer 3 mL specimen to a sterile container. (Min: 0.5 mL) Also acceptable: Transfer to 3 mL viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at 800-522-2787.
Swab or Tissue: Place in 3 mL viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at 800-522-2787.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Bone marrow, CSF, or whole blood. Calcium alginate, eSwab, dry or wood swabs.

Remarks

Specimen source preferred.

Stability

Ambient: 4 hours; Refrigerated: 72 hours; Frozen: Unacceptable

Reference Interval

Negative

Interpretive Data



Compliance Category

Modified FDA

Note

If DFA is negative or inadequate, then an HSV culture will be added. Additional charges apply.

Sensitivity of DFA methodology is dependent upon adequacy of the specimen.  If specimen contains fewer than 20 cells, then the DFA result will be reported as "inadequate."

Hotline History

N/A

CPT Codes

87274; 87273; if reflexed, add 87255

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Components

Component Test Code* Component Chart Name LOINC
0060292 Herpes Simplex Virus 1, DFA 5846-1
0060293 Herpes Simplex Virus 2, DFA 5849-5
0060332 HSVFAC Source 31208-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Herpes Culture
  • Herpes Simplex Viral Culture
  • Herpes Simplex Virus Antigen Detection, DFA
  • Herpes Simplex Virus by DFA
  • HHV1
  • HHV2
  • HSV DFA
  • HSV-1
  • HSV-2
Herpes Simplex Virus DFA with Reflex to Herpes Simplex Virus Culture