Ordering Recommendation
A rapid diagnostic test with culture backup for negative DFA results. Test may be helpful in identifying acute herpes simplex virus (HSV) infection in active lesions (eg, vesicles, ulcers, inflammation of mucous membranes). Molecular testing is generally preferred (refer to Herpes Simplex Virus by PCR (0060041)).
Mnemonic
Methodology
Direct Fluorescent Antibody Stain/Cell Culture
Performed
Sun-Sat
Reported
Within 24 hours
New York DOH Approval Status
Specimen Required
Buccal mucosa lesion, cervical or genital lesion, eye, throat or vesicle swab, bronchoalveolar lavage (BAL), tissue or vesicle fluid.
Fluid: Transfer 3 mL specimen to a sterile container. (Min: 0.5 mL) Also acceptable: Transfer to 3 mL viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Swab or Tissue: Place in 3 mL viral transport media (ARUP Supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Refrigerated.
Bone marrow, CSF, or whole blood. Calcium alginate, eSwab, dry, or wood swabs.
Specimen source preferred.
Ambient: 4 hours; Refrigerated: 72 hours; Frozen: Unacceptable
Reference Interval
Negative
Interpretive Data
FDA
Note
If DFA is negative or inadequate, then a HSV culture will be added. Additional charges apply.
Sensitivity of DFA methodology is dependent upon adequacy of the specimen. If specimen contains fewer than 20 cells, then the DFA result will be reported as "inadequate."
Hotline History
CPT Codes
87274; 87273; if reflexed, add 87252; 87253
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
0060292 | Herpes Simplex Virus 1, DFA | 5846-1 |
0060293 | Herpes Simplex Virus 2, DFA | 5849-5 |
0060332 | HSVFAC Source | 31208-2 |
Aliases
- Herpes Culture
- Herpes Simplex Viral Culture
- Herpes Simplex Virus Antigen Detection, DFA
- Herpes Simplex Virus by DFA
- HHV1
- HHV2
- HSV DFA
- HSV-1
- HSV-2