Amoeba Calcofluor Stain
Ordering Recommendation
Detect Acanthamoeba spp, Naegleria fowleri and other free-living amoebae.
New York DOH Approval Status
Specimen Required
Corneal scrapings or vitreous fluid.
Place corneal scrapings or vitreous fluid in 2 ml of Page's amoeba saline (ARUP supply #31917). Available online through eSupply using ARUP Connect™ or contact Client Services at (800) 522-2787.
Room temperature.
Specimens in media or preservatives. Dry specimens.
Ambient: 72 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Methodology
Qualitative Calcofluor Stain/Microscopy
Performed
Sun-Sat
Reported
Within 24 hours
Reference Interval
Negative
Interpretive Data
This stain will detect free-living amoeba such as Acanthamoeba species, Naegleria fowleri and Balamuthia mandrillaris.
Standard
Note
For CSF specimens refer to Acanthamoeba and Naegleria Culture and Stain, CSF (ARUP Test code 3000878). For Entamoeba histolytica Antigen, EIA (ARUP test code 0058001).
Hotline History
CPT Codes
87206
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
0060250 | Amoeba, Calcofluor Stain | 9781-6 |
Aliases
- Acanthamoeba Stain
- Amoeba calcofluor white
- Naegleria Stain
- Stain for Balamuthia mandrillaris