Ordering Recommendation

Detect Acanthamoeba spp, Naegleria fowleri and other free-living amoebae.

Mnemonic
ACANTSTAIN
Methodology

Qualitative Calcofluor Stain/Microscopy

Performed

Sun-Sat

Reported

Within 24 hours

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Corneal scrapings or vitreous fluid.

Specimen Preparation

Place corneal scrapings or vitreous fluid in 2 ml of Page's amoeba saline (ARUP supply #31917). Available online through eSupply using ARUP Connect™ or contact Client Services at (800) 522-2787.

Storage/Transport Temperature

Room temperature.

Unacceptable Conditions

Specimens in media or preservatives. Dry specimens.

Remarks
Stability

Ambient: 72 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval

Negative

Interpretive Data

This stain will detect free-living amoeba such as Acanthamoeba species, Naegleria fowleri and Balamuthia mandrillaris.

Compliance Category

Standard

Note

For CSF specimens refer to Acanthamoeba and Naegleria Culture and Stain, CSF (ARUP Test code 3000878). For Entamoeba histolytica Antigen, EIA (ARUP test code 0058001).

Hotline History
N/A
CPT Codes

87206

Components
Component Test Code* Component Chart Name LOINC
0060250 Amoeba, Calcofluor Stain 9781-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Acanthamoeba Stain
  • Amoeba calcofluor white
  • Naegleria Stain
  • Stain for Balamuthia mandrillaris
Amoeba Calcofluor Stain