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Time Sensitive
Ordering Recommendation

May provide definitive diagnosis, but sensitivity is low and culture may take several weeks to yield result.

Mnemonic
MC LEPTO
Methodology

Culture

Performed

Sun-Sat

Reported

1-43 days
Positives are reported as soon as detected.
Final: Negative at 6 weeks

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

CSF or blood should be collected within first week of illness; urine after the first week of illness. Blood is collected per blood culture procedure.

Collect

Green (sodium heparin) or sodium oxalate. Also acceptable: Bactec aerobic resin bottle or other commercial blood culture bottles, CSF, or urine.

Specimen Preparation

Blood: Transport 1 mL whole blood in heparin, sodium oxalate tube, or blood culture bottles. (Min: 1 mL)
CSF:
Transport 0.5 mL CSF in a sterile container. (Min: 0.1 mL)
Urine:
Dilute 1 mL urine 1:10 in 1 percent bovine serum albumin within one hour of collection. Transport 4 mL diluted urine in a sterile container. (Min: 0.1 mL)

Storage/Transport Temperature

Room temperature.

Unacceptable Conditions

Clotted whole blood or whole blood submitted in anticoagulant other than those listed above. Urine not diluted with bovine serum albumin within 1 hour of collection or urine in preservative.

Remarks

Specimen source required.

Stability

Blood culture bottles: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Other: Ambient 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval

Culture negative for Leptospira.

Interpretive Data



No compliance statements are in use for this test.

Note

Identification tests are billed separately from culture.

Leptospira spp. may not survive extended transport time or in acidic urine.

Hotline History
N/A
CPT Codes

87081; 87166

Components
Component Test Code* Component Chart Name LOINC
0060158 Culture, Leptospira 594-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Culture, Leptospira
Leptospira Culture