Ordering Recommendation

Monitor respiratory specimens in previously diagnosed patients. Should NOT be ordered without culture in previously undiagnosed patients. For panel test that includes culture and stain, refer to Acid-Fast Bacillus (AFB) Culture and AFB Stain (0060152).

Mnemonic
MS AFB
Methodology

Auramine O Stain

Performed

Sun-Sat

Reported

Within 24 hours

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Sputum or other respiratory specimen.

Specimen Preparation

Transfer 5-10 mL specimen to a sterile container (Min: 1 mL). Place each specimen in an individually sealed bag.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Non-respiratory specimens. Swabs from any sites. Prepared slides.

Remarks

Specimen source required.

Stability

Ambient: 24 hours; Refrigerated: 1 week; Frozen: 2 weeks

Reference Interval

Negative for acid-fast bacilli.

Interpretive Data



Compliance Category

Standard

Note

An AFB Stain alone without culture is appropriate only when following respiratory specimens on previously diagnosed patients. Studies have shown that AFB stain without culture has a poor negative predictive value. For a higher predictive value, order AFB culture (Acid-Fast Bacillus (AFB) Culture, ARUP test code 0060152), which adds an AFB stain.

Hotline History
N/A
CPT Codes

87206; if concentration performed, add 87015

Components
Component Test Code* Component Chart Name LOINC
0060151 Stain, Acid Fast Bacilli 72357-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • AO stain
  • Auramine-rhodamine
  • Kinyoun stain
  • Mycobacteriaum species
Acid-Fast Bacillus (AFB) Stain Only