Ordering Recommendation

Detect and quantify HHV6 subtypes A and B in immunocompromised patients.

Mnemonic
HHV6PCR
Methodology

Quantitative Polymerase Chain Reaction

Performed

Tue-Sat

Reported

1-4 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Lavender (EDTA), pink (K2EDTA), serum separator tube, or CSF.

Specimen Preparation

Separate serum or plasma from cells. Transfer 1 mL serum, plasma or CSF to a sterile container. (Min: 0.5 mL)

Storage/Transport Temperature

Frozen.

Unacceptable Conditions

Heparinized specimens.

Remarks

Specimen source required.

Stability

Ambient: 24 hours; Refrigerated: 5 days; Frozen: 3 months

Reference Interval

Not detected

Interpretive Data

The quantitative range of this test is 3.0-6.0 log copies/mL (1,000-999,000 copies/mL)

A negative result (less than 3.0 log copies/mL or less than 1,000 copies/mL) does not rule out the presence of PCR inhibitors in the patient specimen or HHV6 DNA in concentrations below the level of detection of the test. Inhibition may also lead to underestimation of viral quantitation.

No international standard is currently available for calibration of this test. Caution should be taken when interpreting results generated by different methodologies.

Compliance Statement A: For laboratory developed tests using a manufacturer labeled ASR as the reagent providing the specificity of the assay. Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

Note

The limit of quantification for this DNA assay is 3.0 log copies/mL (1,000 copies/mL). If the assay DID NOT DETECT the virus, the test result will be reported as "<3.0 log copies/mL (<1,000 copies/mL)." If the assay DETECTED the presence of the virus but was not able to accurately quantify the number of copies, the test result will be reported as "Not Quantified."

This assay detects and quantifies HHV6 subtypes A and B.

Hotline History
N/A
Components
Component Test Code* Component Chart Name LOINC
0060072 HHV6 by PCR Source 31208-2
0060073 HHV6 by PCR Type 38348-9
0060075 HHV6 Quant by PCR (copy/mL) 38349-7
0060077 HHV6 Quant by PCR (log copy/mL) 38350-5
0060787 HHV6 by PCR Interp 51730-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • herpes
  • HHV-6 quantitative
  • HHV-6A
  • HHV-6B
  • HHV6 Quant PCR
  • Human herpesvirus 6
  • Roseola
  • Viral load monitoring
Human Herpesvirus 6 (HHV-6A and HHV-6B) by Quantitative PCR