Human Herpesvirus 6 (HHV-6A and HHV-6B) by Quantitative PCR

0060071
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Detected
Not Detected

Ordering Recommendation

Detect and quantify HHV6 subtypes A and B in immunocompromised patients.

Mnemonic

HHV6PCR

Methodology

Quantitative Polymerase Chain Reaction

Performed

Tue-Sat

Reported

1-4 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Lavender (EDTA), pink (K2EDTA), serum separator tube, or CSF.

Specimen Preparation

Separate serum or plasma from cells. Transfer 1 mL serum, plasma or CSF to a sterile container. (Min: 0.5 mL)

Storage/Transport Temperature

Frozen.

Unacceptable Conditions

Heparinized specimens, tissues in optimal cutting temperature compound.

Remarks

Specimen source required.

Stability

Ambient: 24 hours; Refrigerated: 5 days; Frozen: 3 months

Reference Interval

Not detected

Interpretive Data

The quantitative range of this assay is 3.0-6.0 log copies/mL (1,000-999,000 copies/mL).

A negative result (less than 3.0 log copies/mL or less than 1,000 copies/mL) does not rule out the presence of PCR inhibitors in the patient specimen or HHV6 DNA in concentrations below the level of detection of the assay. Inhibition may also lead to underestimation of viral quantitation.

Caution should be taken when interpreting results generated by different assay methodologies.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the U.S. Food and Drug Administration. This test was performed in a CLIA-certified laboratory and is intended for clinical purposes.

Compliance Category

Analyte Specific Reagent (ASR)

Note

The limit of quantification for this DNA assay is 3.0 log copies/mL (1,000 copies/mL). If the assay DID NOT DETECT the virus, the test result will be reported as "<3.0 log copies/mL (<1,000 copies/mL)." If the assay DETECTED the presence of the virus but was not able to accurately quantify the number of copies, the test result will be reported as "Not Quantified."

This assay detects and quantifies HHV6 subtypes A and B.

Hotline History

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Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
02/01/2021
X
N/A

CPT Codes

87533

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Components

Component Test Code* Component Chart Name LOINC
0060072 HHV6 by PCR Source 31208-2
0060073 HHV6 by PCR Type 38348-9
0060075 HHV6 Quant by PCR (copy/mL) 38349-7
0060077 HHV6 Quant by PCR (log copy/mL) 38350-5
0060787 HHV6 by PCR Interp 51730-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • herpes
  • HHV-6 quantitative
  • HHV-6A
  • HHV-6B
  • HHV6 Quant PCR
  • Human herpesvirus 6
  • Roseola
  • Viral load monitoring
Human Herpesvirus 6 (HHV-6A and HHV-6B) by Quantitative PCR